http://hdl.handle.net/11336/385 Articulos de INST.DE INVEST.MEDICAS Mon, 08 Apr 2024 13:55:02 GMT 2024-04-08T13:55:02Z http://hdl.handle.net/11336/230449 Diferencia de género en estrategias de afrontamiento tras situaciones traumáticas de robo; Gender differences in coping strategies after traumatic robbery experiences Serafini, Matías; Sánchez Beisel, Jessica Mariel Frente a una situación estresante, las personas despliegan distintas estrategias de afrontamiento. El afrontamiento se manifiesta como esfuerzos cognitivos o conductuales para reducir el estrés psicológico. Las estrategias de afrontamiento se pueden clasificar en respuestas de aproximación y de evitación, en función de la orientación. La situación de robo es un evento traumático que promueve la expresión de respuestas de afrontamiento orientadas a la evitación, lo cual puede generar síntomas de estrés postraumático. En este trabajo se analizaron las estrategias de afrontamiento en víctimas de robo con los objetivos de evidenciar si estas dependen del contexto de desarrollo (robo en vía pública o en ámbito privado) y analizar si existen diferencias en función del género. Se evaluaron las respuestas de adultos de entre 18 y 55 años mediante el inventario de respuestas de afrontamiento para adultos de Moos. Si bien no se hallaron diferencias en función del contexto de desarrollo, sí se observaron según el género. Mientras que predominan respuestas de aproximación de análisis lógico y respuestas evitativas de aceptación-resignación en el género masculino, el género femenino se caracteriza por respuestas de aproximación basadas en la búsqueda de apoyo. El predominio de esta estrategia es lo que podría hacer al género femenino más receptivo a los tratamientos psicoterapéuticos y favorecer la recuperación del estrés postraumático. Únicamente en el género masculino se encontró una prevalencia en estrategias de afrontamiento evitativas. El análisis de las respuestas de afrontamiento en víctimas de robo puede aportar valor para el estudio de posibles intervenciones terapéuticas.; Faced with a stressful situation, people display different coping strategies. Coping manifests as cognitive or behavioral efforts to reduce psychological stress. Coping strategies can be classified into responses of approach and avoidance, depending on the orientation. Burglary situations are traumatic events that promote the expression of avoidance-oriented coping responses, which can generate symptoms of post-traumatic stress. In this work, coping strategies in burglary victims were analyzed in order to show whether they depend on the development context (burglary on public roads or in the private sphere) and to analyze if there are any differences according to gender. Adults between 18 and 55 years old were assessed using the Moos Adult Coping Response Inventory. No differences were found according to the development context. Significant differences were observed according to gender. While approximation responses of logical analysis and avoidance responses of acceptance-resignation predominate in the male gender, the female gender is characterized by approximation responses based on the search for support. The predominance of this strategy is what could make the female gender more receptive to psychotherapeutic treatments and favor recovery from post-traumatic stress. Only in males, a prevalence of avoidance coping strategies was found. The analysis of the coping responses in burglary victims can provide value for the study of possible therapeutic interventions. Tue, 01 Mar 2022 00:00:00 GMT http://hdl.handle.net/11336/230449 2022-03-01T00:00:00Z http://hdl.handle.net/11336/227802 Total and Extracellular Vesicle cAMP Contents in Urine Are Associated with Autosomal Dominant Polycystic Kidney Disease (ADPKD) Progression Rosenberg, María Lucía; Yaneff, Agustín; Ferradás, Gonzalo Manuel; Villafañe Tapia, Margarita Paz; Davio, Carlos Alberto; Goette, Nora Paula; Vlachovsky, Sandra Gabriela; Peroni, Roxana Noemi; Oddo, Elisabet Mónica; Azurmendi, Pablo Javier ADPKD is the most common genetic renal disease, characterized by the presence of multiple cysts which, through slow and gradual growth, lead to glomerular filtration rate (GFR) decline and end-stage renal disease. Cystic growth is associated with increased intracellular levels of 3′,5′-cyclic adenosine monophosphate (cAMP). Extracellular vesicles (EVs) are proposed to participate in “remote sensing” by transporting different cargoes, but their relevance to ADPKD progression is poorly understood. This study aimed to determine whether cAMP is contained in urinary EVs and, if so, how total and/or EV cAMP contents participate in disease progression. Fourteen ADPKD patients, naïve for V2 receptor antagonism treatment, and seven controls were studied. Progression was evaluated by estimating GFR (eGFR) and height-adjusted total kidney volume (htTKV). Fresh morning urine was collected to determine cAMP by the competitive radioligand assay. Urine EVs were isolated using an adapted centrifugation method and characterized by electron microscopy, dynamic light scanning, flow cytometry with FITC CD63 labeling, protein and RNA content, and AQP2 and GAPDH mRNA detection. Total and EV cAMP was measurable in both control and patient urine samples. Total cAMP was significantly correlated with eGFR and its annual change but inversely correlated with htTKV. The cAMP-EVs showed a bimodal pattern with htTKV, increasing to ~1 L/m and falling at larger sizes. Our results demonstrate that urine cAMP correlates with ADPKD progression markers, and that its extracellular delivery by EVs could reflect the architectural disturbances of the organ. Fri, 01 Sep 2023 00:00:00 GMT http://hdl.handle.net/11336/227802 2023-09-01T00:00:00Z http://hdl.handle.net/11336/225067 NCoA3 upregulation in breast cancer-associated adipocytes elicits an inflammatory profile Lira, María Cecilia; Rosa, Francisco Damián; Aiello, Ignacio; Soares Machado, Mileni; Palma, Alejandra Graciela; Paz, Leonardo Mariano; Salazar Güemes, María Cecilia; Burlando, Silvia; Azurmendi, Pablo Javier; Paladino, Natalia; Costas, Monica Alejandra; Rubio, Maria Fernanda Nuclear receptor coactivator 3 (NCoA3) is a transcriptional coactivator of NF-κB and other factors, which is expressed at relatively low levels in normal cells and is amplified or overexpressed in several types of cancer, including breast tumors. NCoA3 levels have been shown to be decreased during adipogenesis; however, its role in tumor-surrounding adipose tissue (AT) remains unknown. Therefore, the present study assessed the modulation of NCoA3 in breast cancer-associated adipocytes and evaluated its association with the expression of inflammatory markers. 3T3-L1 adipocytes were stimulated with conditioned medium from human breast cancer cell lines and the expression levels of NCoA3 were evaluated by reverse transcription-quantitative (q)PCR. NF-κB activation was measured by immunofluorescence, and tumor necrosis factor and monocyte chemoattractant protein 1 levels were analyzed by qPCR and dot blot assays. The results obtained from the in vitro model were supported using mammary AT (MAT) from female mice, MAT adjacent to tumors from patients with breast cancer and bioinformatics analysis. The results revealed that adipocytes expressing high levels of NCoA3 were mainly associated with a pro-inflammatory profile. In 3T3-L1 adipocytes, NCoA3 downregulation or NF-κB inhibition reversed the expression of inflammatory molecules. In addition, MAT from patients with a worse prognosis exhibited high levels of this coactivator. Notably, adipocyte NCoA3 levels could be modulated by inflammatory signals from tumors. The modulation of NCoA3 levels in synergy with NF-κB activity in MAT in a tumor context could be factors required to establish breast cancer-associated inflammation. As adipocytes are involved in the development and progression of breast cancer, this signaling network deserves to be further investigated to improve future tumor treatments. Mon, 01 May 2023 00:00:00 GMT http://hdl.handle.net/11336/225067 2023-05-01T00:00:00Z http://hdl.handle.net/11336/224150 Personalized medicine in nonalcoholic fatty liver disease Pirola, Carlos Jose; Sookoian, Silvia Cristina The keystone of precision medicine in complex traits is to predict the onset and progression of the disease. While there are different paths toward precision medicine,1 genetic assessment may be among the top strategies. The current knowledge of the complete human genome sequence and the availability of next-generation sequencing technologies2 to decipher individual patients’ genetic make-up also invite fully integrated genomic medicine. Therefore, patients may be classified into high or low risk for disease onset or severity, and therapeutic interventions can be personalized. Most importantly, precision medicine must ensure health benefits across geography, ethnicity, age, and gender... Wed, 01 Jun 2022 00:00:00 GMT http://hdl.handle.net/11336/224150 2022-06-01T00:00:00Z