http://hdl.handle.net/11336/262 Articulos de INST.DE INVEST.PARA LA INDUSTRIA QUIMICA (I) Wed, 13 Mar 2024 22:18:01 GMT 2024-03-13T22:18:01Z http://hdl.handle.net/11336/230421 Evaluation of edible films as single-serve pouches for a sustainable packaging system Gamboni, Jimena Elizabeth; Bonfiglio, Gisella Vanesa; Slavutsky, Anibal Marcelo; Bertuzzi, Maria Alejandra This research aims to evaluate a sustainable alternative that allows reducing unnecessary plastic waste through the design of a packaging system for dry food. This consists of a primary package for single-serve soluble edible pouches and a secondary package to provide protection against excessive moisture and the necessary protection during transportation, storage, and marketing. Thus, the objective of this research is to assess a methodology that allows the design of a system that combines both, biopolymeric and synthetic materials taking into consideration the product shelf-life estimation. Instant coffee was selected as the dry food to evaluate due to its high consumption and popularity. The sorption isotherms of the coffee samples were obtained. The primary packaging was made from gelatin selected between starch and pectin. Tensile strength and heat sealing resistance were assessed. From the data obtained and thermodynamic analysis, the shelf life of the product was analytically estimated. The performance of the system was analyzed by simulating the daily consumption of 30 individual soluble pouches and a sensory analysis was performed to determine whether panelists could detect the presenceof the pouch. The proposed packaging system is suitable for packaging individual coffee pouches since it preserves the quality characteristics of the product. Fri, 01 Dec 2023 00:00:00 GMT http://hdl.handle.net/11336/230421 2023-12-01T00:00:00Z http://hdl.handle.net/11336/230408 Antibacterial Activity of Bacillus Lipopeptides Vehiculized and Delivered by Biopolymeric Films Colodro, María Verónica; Torres, María Julia; Slavutsky, Anibal Marcelo; Audisio, Marcela Carina; Bertuzzi, Maria Alejandra Active films based on different biopolymers (pectin, Brea gum, carboxymethylcellulose, and composite starch-gelatin) were prepared by casting. Lipopeptides synthesized by bacteria of the Bacillus genus were added as antimicrobial active agent. Antibacterial activity was evaluated and quantified by direct contact technique against different strains of Listeria monocytogenes, Escherichia coli, and Salmonella enterica sp. Incorporation of lipopeptides did not cause significant changes in mechanical and barrier properties of films. Antibacterial effect of functionalized films depended on the nature of the polymeric matrix and the pathogen studied. Functionalized pectin films inhibited the growth of all strains studied, with non-functionalized pectin films also showing inhibition of L. monocytogenes. Functionalized starch-gelatin films only inhibited the growth of L. monocytogenes. The other matrices did not show adequate performance for this type of application. The results suggested that pectin and starch-gelatin composite can be used as effective lipopeptide carriers to produce antimicrobial active films for food preservation. Thu, 01 Jun 2023 00:00:00 GMT http://hdl.handle.net/11336/230408 2023-06-01T00:00:00Z http://hdl.handle.net/11336/230397 Parásitos humanos en aguas superficiales de uso recreativo en Salta, Argentina; Human parasites in surface water used for recreation in Salta, Argentina Sanguino Jorquera, Diego Gastón; Poma, Hugo Ramiro; Rajal, Verónica Beatriz; Juarez, Maria Mercedes; Irazusta, Verónica Patricia Las enfermedades transmitidas por el agua pueden tener diferente origen; las bacterias y los parásitos son los agentes causales más importantes. En este trabajo se monitorearon muestras de agua en tres puntos próximos a una zona recreativa en la provincia de Salta (Argentina): dos en un arroyo que rodea a un complejo termal, aguas arriba y aguas abajo de donde descargan las aguas de dicho complejo; el tercero, en la zona de salida del agua proveniente del complejo. Las muestras se tomaron en cuatro meses del año. Se realizó la caracterización fisicoquímica, bacteriológica y búsqueda de parásitos y amebas de vida libre (AVL) del agua en cada punto. Las muestras de agua a la salida del complejo termal presentaron características fisicoquímicas diferentes a las del arroyo. En todos los puntos se detectaron indicadores bacterianos de contaminación, pero el agua del arroyo tuvo una concentración significativamente mayor de Pseudomonas sp. En las muestras del arroyo se encontraron, esporádicamente, huevos de Ascaris spp., quistes de Giardia sp. y huevos de ancilostomideos. En todas las muestras se encontraron AVL; se aislaron 15 AVL, que se identificaron como Acanthamoeba sp., mayoritariamente pertenecientes al genotipo T4. La vigilancia de parásitos en ambientes acuáticos de uso recreativo es un complemento importante a la de los indicadores microbiológicos tradicionales para la evaluación de la calidad del agua. Los parásitos identificados representan un potencial riesgo para la salud de las personas que hacen uso de dichos ambientes.; Waterborne diseases can have different origins, micro-organisms such as bacteria and parasites being the most important ones. In this study, two recreational aquatic environments were studied in the province of Salta, Argentina. Water samples collected from three different locations, two from a creek and one from the outlet of a thermal complex, were monitored at four time points. Physicochemical and microbiological characterization of each point was conducted, as well as a search for parasites and amebae. Parasites were identified through optical microscopy observations and free-living amebae (FLA) were isolated by spiking in Petri dishes followed by subsequent molecular identification. Water samples from the outlet of the thermal complex showed different physicochemical characteristics from those of the creek. Bacterial indicators of contamination were detected at all points; however, the creek water had a significantly higher concentration of Pseudomonas sp. Sporadically, creek samples exhibited Ascaris spp. eggs, Giardia sp. cysts, and ancylostomid eggs. The presence of FLA was observed in all samples, 15 of which were isolated and identified as Acanthamoeba sp., mostly belonging to the T4 genotype. Parasite surveillance in recreational aquatic environments is an important complement to traditional microbial indicators for assessing water quality. The identified parasites represent a potential health risk for people using these environments. Fri, 01 Dec 2023 00:00:00 GMT http://hdl.handle.net/11336/230397 2023-12-01T00:00:00Z http://hdl.handle.net/11336/230357 Application of an edible coating developed with Andean potato starch and carboxymethyl-cellulose for lipid reduction during frying; Aplicación de un recubrimiento comestible desarrollado con almidón de papa andina y carboximetilcelulosa para la reducción de lípidos durante la fritura Calliope, Sonia Rosario; Slavutsky, Anibal Marcelo; Segura, N.; Samman, Norma Cristina Este trabajo tuvo como objetivo buscar alternativas de uso para diferentes variedades de papas andinas (Solanum tuberosum ssp andigenum) reintroducidas en el noroeste argentino. Específicamente, el desarrollo de películas simples y compuestas elaboradas con hidrocoloides como la carboximetilcelulosa (CMC) y el almidón (S) extraído de papa andina variedad Runa, y su aplicación como cobertura en frituras de papas andinas var. Waycha fue estudiado para minimizar la absorción de aceite. También se evaluó el efecto del blanqueo previo de las hojuelas con diferentes medios: agua, solución de cloruro de calcio y ácido ascórbico. Los recubrimientos se aplicaron a patatas fritas antes de freírlas. Los resultados mostraron que el tipo de aceite utilizado no afectó su absorción por parte de las hojuelas. Los tratamientos de blanqueo con cloruro de calcio y recubrimiento con S/CMC, mostraron una reducción significativa en la absorción de aceite (39,5 % ± 0,7), retrasaron su oxidación y disminuyeron la pérdida de tocoferoles durante el proceso de fritura. También contribuyó a las características físicas y sensoriales del producto final, que presentó alta aceptabilidad por parte de los consumidores.; This work aimed to search for alternative uses for different varieties of Andean potatoes (Solanum tuberosum ssp andigenum) reintroduced in north-western Argentina. Specifically, the development of simple and compounds films made with hydrocolloids such as carboxymethyl-cellulose (CMC) and starch (S) extracted from Andean potatoes var. Runa, and its application as a cover in deep frying of Andean potato chips var. Waycha was studied to minimize oil absorption. The effect of prior bleaching of the chips with different media was also evaluated: water, calcium chloride solution, and ascorbic acid. The coatings were applied to potatoes chips before being fried. The results showed that the type of oil used did not affect their absorption by the chips. The bleaching treatments with calcium chloride and coating with S/CMC, showed a significant reduction in the oil absorption (39.5 % ± 0.7), delayed its oxidation, and decreased the loss of tocopherols during the frying process. It also contributed to the physical and sensory characteristics of the final product, which presented high acceptability by consumers. Thu, 01 Jun 2023 00:00:00 GMT http://hdl.handle.net/11336/230357 2023-06-01T00:00:00Z