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Ana Maite Folle, Eduardo S. Kitano, Analía Lima, Magdalena Gil, Marcela Cucher, Gustavo Mourglia-Ettlin, Leo K. Iwai, Mara Rosenzvit, Carlos Batthyány, Ana María Ferreira
Characterisation of Antigen B Protein Species Present in the Hydatid Cyst Fluid of Echinococcus canadensis G7 Genotype
www.plosntds.org
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2.0012 0 Td
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(achieved)Tj
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(or)Tj
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(dis-)Tj
-35.0586 -1.2982 Td
(ease)Tj
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1.0374 0 Td
(others)Tj
2.7099 0 Td
(\(reviewed)Tj
4.1329 0 Td
(by)Tj
1.1849 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(])Tj
2.075 0 Td
(and)Tj
1.7291 0 Td
([)Tj
0.83 0.64 0.02 0 k
(9)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(]\).)Tj
3.4129 0 Td
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4.3426 0 Td
(CE)Tj
1.4513 0 Td
(diagnosis,)Tj
4.184 0 Td
(antigen)Tj
3.2031 0 Td
(B)Tj
0.805 0 Td
(\(AgB\),)Tj
2.8517 0 Td
(an)Tj
-34.9793 -1.2983 Td
(abundant)Tj
4.0421 0 Td
(parasite)Tj
3.3279 0 Td
(component)Tj
4.8132 0 Td
(present)Tj
3.1634 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(HF)Tj
1.5137 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F13 1 Tf
1.474 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
0.9922 0 Td
[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
/F8 1 Tf
4.422 0 Td
(s.l.)Tj
1.2812 0 Td
(metacestode,)Tj
5.3858 0 Td
(is)Tj
0.8447 0 Td
(the)Tj
-34.7808 -1.3039 Td
(most)Tj
2.2053 0 Td
(immunogenic)Tj
5.8393 0 Td
(and)Tj
1.7291 0 Td
(specific)Tj
/F13 1 Tf
3.1918 0 Td
[()-1()13()18()12()6()4()7()4()7()1()-4()]TJ
/F8 1 Tf
(genus)Tj
7.9994 0 Td
(antigen.)Tj
3.4299 0 Td
(It)Tj
0.856 0 Td
(is)Tj
0.8504 0 Td
(a)Tj
0.652 0 Td
(230)Tj
1.6497 0 Td
(kDa)Tj
1.8822 0 Td
(lipoprotein)Tj
4.6942 0 Td
(that)Tj
-34.9793 -1.2983 Td
(carries)Tj
2.8686 0 Td
(a)Tj
0.6576 0 Td
(huge)Tj
2.16 0 Td
(amount)Tj
3.3562 0 Td
(of)Tj
1.0148 0 Td
(both)Tj
2.0636 0 Td
(neutral)Tj
3.0671 0 Td
(and)Tj
1.7291 0 Td
(polar)Tj
2.2961 0 Td
(lipids)Tj
2.4094 0 Td
(\(around)Tj
3.4752 0 Td
(50%)Tj
1.9276 0 Td
(in)Tj
1.0375 0 Td
(mass\))Tj
2.5341 0 Td
(including)Tj
4.0422 0 Td
(fatty)Tj
-34.6391 -1.2982 Td
(acids)Tj
2.2336 0 Td
(\(FA\))Tj
2.109 0 Td
(and)Tj
1.7291 0 Td
(sterols,)Tj
3.0161 0 Td
(which)Tj
/F13 1 Tf
2.6475 0 Td
[()-1()13()18()12()6()4()7()4()7()1()-4()]TJ
/F8 1 Tf
5.3461 0 Td
(is)Tj
0.8504 0 Td
(not)Tj
1.5704 0 Td
(capable)Tj
3.2088 0 Td
(of)Tj
1.0148 0 Td
(synthesising)Tj
5.1023 0 Td
(\(reviewed)Tj
4.1329 0 Td
(by)Tj
1.1849 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(]\).)Tj
-34.1459 -1.2983 Td
(This)Tj
1.9842 0 Td
(has)Tj
1.542 0 Td
(led)Tj
1.4173 0 Td
(to)Tj
1.0262 0 Td
(emphasise)Tj
4.354 0 Td
(its)Tj
1.1508 0 Td
(hypothetical)Tj
5.142 0 Td
(role)Tj
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(in)Tj
1.0375 0 Td
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3.3222 0 Td
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(metabolism,)Tj
5.1307 0 Td
(taking)Tj
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(up)Tj
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(host)Tj
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(lip-)Tj
-35.8296 -1.3039 Td
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(building)Tj
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(blocks)Tj
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4.1442 0 Td
(demands.)Tj
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4.3937 0 Td
(this)Tj
1.6781 0 Td
(hypothesis)Tj
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(is)Tj
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-33.0574 -1.2983 Td
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1.1792 0 Td
(the)Tj
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(that)Tj
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(AgB)Tj
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(belongs)Tj
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(a)Tj
0.652 0 Td
(cestode-specific)Tj
6.5026 0 Td
(family)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(proteins)Tj
3.5149 0 Td
(exhibiting)Tj
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(ability)Tj
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(to)Tj
1.0261 0 Td
(bind)Tj
-34.7411 -1.2983 Td
(hydrophobic)Tj
5.3574 0 Td
(ligands)Tj
3.0671 0 Td
(\(HLBP)Tj
3.0047 0 Td
(for)Tj
1.3889 0 Td
(hydrophobic)Tj
5.3575 0 Td
(ligand)Tj
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(binding)Tj
3.3505 0 Td
(protein\))Tj
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([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
3.2541 0 Td
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1.9843 0 Td
(family)Tj
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-35.6993 -1.3039 Td
(emerged)Tj
3.668 0 Td
(by)Tj
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(gene)Tj
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(expansion)Tj
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(events,)Tj
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(giving)Tj
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(rise)Tj
1.6384 0 Td
(to)Tj
1.0148 0 Td
(species)Tj
2.9934 0 Td
(and)Tj
1.7234 0 Td
(gene-specific)Tj
5.3915 0 Td
(mono-)Tj
-34.8602 -1.2982 Td
(phyletic)Tj
3.3788 0 Td
(clades.)Tj
2.8573 0 Td
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5.4935 0 Td
(HLBP)Tj
2.6646 0 Td
(members)Tj
3.9458 0 Td
(are)Tj
1.4513 0 Td
(all)Tj
1.1452 0 Td
(immunodominant)Tj
7.6592 0 Td
(antigens.)Tj
-27.3995 -1.2983 Td
(AgB)Tj
1.9502 0 Td
(antigenicity)Tj
4.9152 0 Td
(has)Tj
1.5477 0 Td
(been)Tj
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(associated)Tj
4.2577 0 Td
(with)Tj
1.9955 0 Td
(its)Tj
1.1452 0 Td
(protein)Tj
3.1521 0 Td
(moiety)Tj
2.9934 0 Td
(\(apolipoprotein)Tj
6.4856 0 Td
(components\))Tj
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([)Tj
0.83 0.64 0.02 0 k
(17)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(19)Tj
0 g
(])Tj
3.3165 0 Td
(that)Tj
1.7801 0 Td
(is)Tj
0.8504 0 Td
(encoded)Tj
3.6 0 Td
(by)Tj
1.1792 0 Td
(a)Tj
0.6576 0 Td
(multigene)Tj
4.2406 0 Td
(and)Tj
1.7291 0 Td
(polymorphic)Tj
5.3802 0 Td
(family)Tj
2.7269 0 Td
(with)Tj
1.9956 0 Td
(five)Tj
1.6554 0 Td
(AgB)Tj
1.9502 0 Td
(gene)Tj
2.0806 0 Td
(products)Tj
-33.1424 -1.2983 Td
(named)Tj
/F13 1 Tf
2.9706 0 Td
[()37( )]TJ
/F8 1 Tf
2.3244 0 Td
(to)Tj
/F13 1 Tf
1.0205 0 Td
[()37( )]TJ
/F8 1 Tf
2.3244 0 Td
(\(revised)Tj
3.3902 0 Td
(by)Tj
1.1792 0 Td
([)Tj
0.83 0.64 0.02 0 k
(20)Tj
0 g
(]\).)Tj
2.4151 0 Td
(The)Tj
1.7858 0 Td
(recent)Tj
2.7043 0 Td
(assembly)Tj
3.8324 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()-1()13()18()12()6()4()7()4()7()1()-4()]TJ
5.3404 0 Td
[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
/F8 1 Tf
4.422 0 Td
(G1)Tj
-34.7241 -1.2983 Td
(genotype)Tj
3.855 0 Td
(and)Tj
/F13 1 Tf
1.7348 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
0.9922 0 Td
[()18()4()14()7()4()4()4()1()4()4()16(\n)-9()-4()]TJ
/F8 1 Tf
5.6068 0 Td
(genomes)Tj
3.7644 0 Td
(confirmed)Tj
4.3994 0 Td
(that)Tj
1.7801 0 Td
(this)Tj
1.6725 0 Td
(scenario)Tj
3.5546 0 Td
(is)Tj
0.8504 0 Td
(highly)Tj
2.7099 0 Td
(conserved)Tj
-30.9201 -1.2982 Td
(among)Tj
/F13 1 Tf
2.9933 0 Td
[()-1()13()18()12()6()4()7()4()7()1()-4()]TJ
/F8 1 Tf
5.3405 0 Td
(species)Tj
2.999 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.0749 0 Td
(The)Tj
1.7859 0 Td
(mature)Tj
3.0954 0 Td
(protein)Tj
3.1521 0 Td
(products)Tj
3.753 0 Td
(of)Tj
1.0148 0 Td
(these)Tj
2.2564 0 Td
(genes)Tj
2.4378 0 Td
(are)Tj
1.4513 0 Td
(small)Tj
-32.3544 -1.3039 Td
(\(around)Tj
3.4752 0 Td
(8)Tj
0.6973 0 Td
(kDa)Tj
1.8822 0 Td
(in)Tj
1.0375 0 Td
(mass\),)Tj
/F14 1 Tf
2.7609 0 Td
()Tj
/F8 1 Tf
0.788 0 Td
(helix-rich)Tj
4.0932 0 Td
(secreted)Tj
3.4696 0 Td
(polypeptides,)Tj
5.5105 0 Td
(with)Tj
1.9956 0 Td
(ability)Tj
2.6929 0 Td
(to)Tj
1.0262 0 Td
(self-assembly)Tj
5.4935 0 Td
(gener-)Tj
-34.9226 -1.2983 Td
(ating)Tj
2.2336 0 Td
(high-molecular-mass)Tj
8.6797 0 Td
(oligomers)Tj
4.1895 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
(];)Tj
3.2542 0 Td
(they)Tj
1.9219 0 Td
(are)Tj
1.4513 0 Td
(thus)Tj
1.9332 0 Td
(named)Tj
2.9764 0 Td
(AgB8/1)Tj
3.2258 0 Td
(to)Tj
1.0261 0 Td
(AgB8/5)Tj
3.2315 0 Td
(sub-)Tj
-34.1232 -1.2983 Td
(units.)Tj
2.4491 0 Td
(The)Tj
1.7801 0 Td
(native)Tj
2.6476 0 Td
(antigen,)Tj
3.4299 0 Td
(the)Tj
1.4683 0 Td
(recombinant)Tj
5.3688 0 Td
(AgB8/1)Tj
3.2315 0 Td
(and)Tj
1.7291 0 Td
(AgB8/2)Tj
3.2315 0 Td
(subunits,)Tj
3.8437 0 Td
(as)Tj
1.0148 0 Td
(well)Tj
1.8198 0 Td
(as)Tj
1.0148 0 Td
(various)Tj
-33.029 -1.3039 Td
(synthetic)Tj
3.821 0 Td
(peptides)Tj
3.5547 0 Td
(derived)Tj
3.2201 0 Td
(from)Tj
2.2053 0 Td
(them,)Tj
2.5115 0 Td
(have)Tj
2.0636 0 Td
(shown)Tj
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(to)Tj
1.0261 0 Td
(be)Tj
1.1509 0 Td
(valuable)Tj
3.4922 0 Td
(for)Tj
1.3833 0 Td
(CE)Tj
1.457 0 Td
(diagnosis)Tj
3.9572 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(];)Tj
3.5433 0 Td
(all)Tj
-36.2322 -1.2982 Td
(of)Tj
1.0147 0 Td
(them)Tj
2.2904 0 Td
(have)Tj
2.058 0 Td
(shown)Tj
2.8459 0 Td
(similar)Tj
2.9877 0 Td
(diagnostic)Tj
4.32 0 Td
(performance)Tj
5.3461 0 Td
(in)Tj
1.0375 0 Td
(comparison)Tj
4.9776 0 Td
(with)Tj
1.9956 0 Td
(crude)Tj
2.4944 0 Td
(HF)Tj
1.5137 0 Td
(prepara-)Tj
-32.8816 -1.2983 Td
(tions,)Tj
2.4264 0 Td
(but)Tj
1.5534 0 Td
(in)Tj
1.0374 0 Td
(some)Tj
2.3301 0 Td
(clinical)Tj
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(stop)Tj
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(codon)Tj
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(33)Tj
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[()7()16()6()6(\r)4()2()10()-4()]TJ
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[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
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4.422 0 Td
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([)Tj
0.83 0.64 0.02 0 k
(23)Tj
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(,)Tj
0.83 0.64 0.02 0 k
(35)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
(36)Tj
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/F13 1 Tf
2.4945 0 Td
[()]TJ
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(.)Tj
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0.9978 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
/F8 1 Tf
4.4334 0 Td
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1.474 0 Td
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1.0148 0 Td
[()]TJ
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(.)Tj
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0.9921 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
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4.4334 0 Td
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4.0819 0 Td
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1.627 0 Td
(this)Tj
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3.3165 0 Td
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1.8312 0 Td
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(main)Tj
2.2847 0 Td
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-32.2977 -1.2983 Td
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/F13 1 Tf
1.389 0 Td
[()]TJ
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(.)Tj
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0.9921 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
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4.4334 0 Td
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1.4116 0 Td
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4.0819 0 Td
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1.7291 0 Td
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3.1464 0 Td
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3.753 0 Td
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1.1792 0 Td
(the)Tj
1.474 0 Td
(germi-)Tj
-34.5427 -1.3039 Td
(nal)Tj
1.4456 0 Td
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2.1543 0 Td
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1.0148 0 Td
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1.8199 0 Td
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5.8109 0 Td
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5.2441 0 Td
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1.4683 0 Td
(parasite)Tj
3.3222 0 Td
(material)Tj
3.515 0 Td
(where)Tj
2.6532 0 Td
(AgB)Tj
1.9502 0 Td
(accumulates.)Tj
-31.4133 -1.2983 Td
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6.7464 0 Td
(and)Tj
1.7291 0 Td
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6.3609 0 Td
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4.7338 0 Td
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4.0422 0 Td
(two-dimensional)Tj
7.0129 0 Td
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1.3549 0 Td
(electrophore-)Tj
-31.9802 -1.2983 Td
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1.2075 0 Td
(\(2-DGE\))Tj
3.7474 0 Td
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1.7291 0 Td
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2.5625 0 Td
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6.8825 0 Td
(\(LC\))Tj
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3.3959 0 Td
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1.0261 0 Td
(mass)Tj
2.194 0 Td
(spectrometry)Tj
5.4879 0 Td
(\(MS\))Tj
2.2677 0 Td
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2.1259 0 Td
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5.0683 0 Td
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1.0148 0 Td
[()]TJ
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(.)Tj
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0.9921 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
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4.4334 0 Td
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1.8595 0 Td
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2.1487 0 Td
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1.4683 0 Td
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[()]TJ
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[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
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[()7()16()6()6(\r)4()2()10()-4()]TJ
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(AgB8/1)Tj
3.2315 0 Td
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1.9955 0 Td
[()]TJ
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(.)Tj
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0.9978 0 Td
[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
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4.4221 0 Td
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1.3946 0 Td
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0.83 0.64 0.02 0 k
(23)Tj
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0.9921 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
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4.4334 0 Td
(G7)Tj
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1.474 0 Td
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3.3619 0 Td
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1.7745 0 Td
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1.389 0 Td
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5.6296 0 Td
(immunoassays)Tj
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(\(reviewed)Tj
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0.83 0.64 0.02 0 k
(26)Tj
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(the)Tj
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1.627 0 Td
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3.4923 0 Td
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 222.0094 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
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(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
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Q
q
1 j
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BT
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2.7779 0 Td
(hydatid)Tj
3.2541 0 Td
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(24\))Tj
1.5137 0 Td
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3.7417 0 Td
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2.2054 0 Td
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2.3414 0 Td
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3.4185 0 Td
(pigs)Tj
1.8312 0 Td
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2.9253 0 Td
(the)Tj
1.474 0 Td
(routine)Tj
3.1635 0 Td
(work)Tj
2.279 0 Td
(of)Tj
1.0148 0 Td
(local)Tj
2.075 0 Td
(abattoirs)Tj
3.6963 0 Td
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1.0375 0 Td
(Buenos)Tj
3.1691 0 Td
(Aires)Tj
2.3244 0 Td
(\(Argentina\).)Tj
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q
1 j
1 J
0 w
7.4999 0 0 6.6659 302.9102 562.7338 cm
BT
/F8 1 Tf
1 TL
-0.0107 Tc
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(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 308.5795 564.3779 cm
BT
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1 TL
-0.008 Tc
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([)Tj
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1 J
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7.4999 0 0 6.6659 479.9622 536.7118 cm
BT
/F8 1 Tf
1 TL
-0.0107 Tc
0 0 Td
(AgB)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F8 1 Tf
1 TL
-0.008 Tc
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1.3153 0 Td
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2.4094 0 Td
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(and)Tj
1.7178 0 Td
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1.3776 0 Td
(iBAQ,)Tj
2.6986 0 Td
(riBAQ)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 329.4425 497.707 cm
BT
/F8 1 Tf
1 TL
-0.0107 Tc
0 0 Td
(AgB)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 342.2551 499.4078 cm
BT
/F8 1 Tf
1 TL
-0.008 Tc
0 0 Td
(,)Tj
0.4422 0 Td
(score)Tj
2.2847 0 Td
(and)Tj
1.7177 0 Td
(the)Tj
1.4627 0 Td
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4.5071 0 Td
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(\(%)Tj
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1.9615 0 Td
(3)Tj
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1.0092 0 Td
(the)Tj
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(5)Tj
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(analytical)Tj
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(replicates)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 421.1716 cm
BT
/F0 1 Tf
1 TL
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2.296 0 Td
(extraction)Tj
4.5024 0 Td
(and)Tj
1.8662 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 404.2204 cm
BT
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1 TL
0.005 Tc
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(AgB)Tj
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(total)Tj
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Q
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1 j
1 J
0 w
7.4999 0 0 6.6659 358.8094 402.5196 cm
BT
/F8 1 Tf
1 TL
0.0067 Tc
0 0 Td
(f)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 363.2881 404.2204 cm
BT
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1 TL
0.005 Tc
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(0.25)Tj
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1.8142 0 Td
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1.3436 0 Td
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(38)Tj
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1.2019 0 Td
(HPTLC)Tj
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(double)Tj
3.0103 0 Td
(development)Tj
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([)Tj
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0 g
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2.1203 0 Td
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1.5817 0 Td
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2.092 0 Td
(bands)Tj
2.6419 0 Td
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(secondary)Tj
4.3484 0 Td
(standards)Tj
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-34.2479 -1.2983 Td
(same)Tj
2.2904 0 Td
(HPTLC)Tj
3.4072 0 Td
(plate.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 313.0015 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.5023 Td
(Apolipoprotein)Tj
6.5197 0 Td
(composition)Tj
5.4945 0 Td
(of)Tj
1.0441 0 Td
(AgB)Tj
2.093 0 Td
(present)Tj
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0.9874 0 Td
(E)Tj
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(.)Tj
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1.1575 0 Td
(canadensis)Tj
/F0 1 Tf
5.2158 0 Td
(HF)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 278.022 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
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1.7801 0 Td
(apolipoprotein)Tj
6.1511 0 Td
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1.0148 0 Td
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1.9446 0 Td
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3.1634 0 Td
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1.0375 0 Td
(fertile)Tj
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(HF)Tj
1.5137 0 Td
(of)Tj
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1.0148 0 Td
[()]TJ
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(.)Tj
/F13 1 Tf
0.9921 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
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4.439 0 Td
(G7)Tj
1.406 0 Td
(genotype)Tj
3.8608 0 Td
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-36.1018 -1.3039 Td
(analysed)Tj
3.651 0 Td
(using)Tj
2.3867 0 Td
(MS)Tj
1.5874 0 Td
(based)Tj
2.4718 0 Td
(methodologies.)Tj
6.3269 0 Td
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1.6271 0 Td
(this)Tj
1.6724 0 Td
(purpose,)Tj
3.6737 0 Td
(we)Tj
1.3266 0 Td
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3.804 0 Td
(a)Tj
0.6577 0 Td
(biological)Tj
4.0875 0 Td
(represen-)Tj
-33.2728 -1.2982 Td
(tative)Tj
2.3924 0 Td
(pool)Tj
1.9956 0 Td
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/F13 1 Tf
1.0148 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
0.9864 0 Td
[()7()16()6()6(\r)4()2()10()-4()]TJ
/F8 1 Tf
4.4334 0 Td
(HF)Tj
1.5137 0 Td
(from)Tj
2.2053 0 Td
(24)Tj
1.1679 0 Td
(individual)Tj
4.2859 0 Td
(swine)Tj
2.5002 0 Td
(cysts,)Tj
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(G7)Tj
1.4059 0 Td
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1.0261 0 Td
(COX1)Tj
2.7552 0 Td
(genotyping.)Tj
4.9436 0 Td
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4.0762 0 Td
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1.0262 0 Td
(achieve)Tj
3.1747 0 Td
(an)Tj
1.2019 0 Td
(adequate)Tj
3.7928 0 Td
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1.9503 0 Td
(apolipoprotein)Tj
6.1454 0 Td
(charac-)Tj
-34.503 -1.3039 Td
(terisation,)Tj
4.2179 0 Td
(we)Tj
1.3323 0 Td
(firstly)Tj
2.5058 0 Td
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(out)Tj
1.5534 0 Td
(an)Tj
1.2018 0 Td
(enrichment)Tj
4.8699 0 Td
(step)Tj
1.8199 0 Td
(since)Tj
2.245 0 Td
(AgB)Tj
1.9502 0 Td
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0.8447 0 Td
(poorly)Tj
2.8233 0 Td
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1.0375 0 Td
(HF)Tj
-34.35 -1.2983 Td
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4.2406 0 Td
(to)Tj
1.0261 0 Td
(host)Tj
1.9162 0 Td
(albumin)Tj
3.5716 0 Td
(and)Tj
1.7292 0 Td
(immunoglobulins.)Tj
7.6081 0 Td
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3.0444 0 Td
(advantage)Tj
4.2406 0 Td
(that)Tj
1.7745 0 Td
(AgB)Tj
1.9502 0 Td
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1.6271 0 Td
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1.1508 0 Td
(selec-)Tj
-33.8794 -1.2982 Td
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4.0082 0 Td
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3.5093 0 Td
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(a)Tj
0.652 0 Td
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3.7757 0 Td
([)Tj
0.83 0.64 0.02 0 k
(40)Tj
0 g
(])Tj
1.8482 0 Td
(or)Tj
1.0998 0 Td
(Q-Sepharose)Tj
5.3575 0 Td
(beads)Tj
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([)Tj
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(41)Tj
0 g
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2.0749 0 Td
(we)Tj
1.3266 0 Td
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3.8097 0 Td
(an)Tj
1.2019 0 Td
(AgB)Tj
1.9502 0 Td
(enriched-fraction)Tj
7.217 0 Td
(by)Tj
1.1849 0 Td
(a)Tj
0.652 0 Td
(single)Tj
2.5341 0 Td
(step)Tj
1.8198 0 Td
(anion)Tj
2.5229 0 Td
(exchange)Tj
3.9288 0 Td
(chromatography)Tj
-30.2228 -1.2982 Td
(of)Tj
1.0147 0 Td
(HF)Tj
1.5137 0 Td
(on)Tj
1.2756 0 Td
(Q-Sepharose;)Tj
5.5842 0 Td
(this)Tj
1.6725 0 Td
(step)Tj
1.8198 0 Td
(concentrates)Tj
5.2837 0 Td
(AgB)Tj
1.9503 0 Td
(favouring)Tj
4.0818 0 Td
(the)Tj
1.4684 0 Td
(detection)Tj
3.9401 0 Td
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1.0148 0 Td
(lower)Tj
2.4491 0 Td
(repre-)Tj
-33.0687 -1.2983 Td
(sented)Tj
2.8006 0 Td
(apolipoproteins.)Tj
0.83 0.64 0.02 0 k
6.735 0 Td
(S1)Tj
1.1736 0 Td
(Fig)Tj
0 g
1.4796 0 Td
(shows)Tj
2.6589 0 Td
(the)Tj
1.4684 0 Td
(SDS-PAGE)Tj
4.7905 0 Td
(analysis)Tj
3.3222 0 Td
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1.0148 0 Td
(fractions)Tj
3.719 0 Td
(obtained)Tj
3.736 0 Td
(by)Tj
1.1792 0 Td
(this)Tj
-34.0778 -1.2983 Td
(chromatography.)Tj
7.1035 0 Td
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1.2643 0 Td
(expected,)Tj
3.9458 0 Td
(AgB)Tj
1.9502 0 Td
(was)Tj
1.7008 0 Td
(retained)Tj
3.5149 0 Td
(by)Tj
1.1792 0 Td
(Q-Sepharose)Tj
5.3575 0 Td
(beads)Tj
2.4718 0 Td
(and)Tj
1.7348 0 Td
(eluted)Tj
2.6645 0 Td
(with)Tj
1.9956 0 Td
(500)Tj
-34.8829 -1.3039 Td
(mM)Tj
1.9332 0 Td
(NaCl)Tj
2.296 0 Td
(pH)Tj
1.508 0 Td
(=)Tj
0.7994 0 Td
(7.4)Tj
1.3946 0 Td
(\(fraction)Tj
3.6964 0 Td
(QS)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 328.5354 133.3417 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(f)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 330.6897 134.9858 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(\))Tj
0.5612 0 Td
(since)Tj
2.2394 0 Td
(QS)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 370.9417 133.3417 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(f)Tj
ET
Q
q
1 j
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