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Pamela Cribb, Virginia Perdomo, Victoria L. Alonso, Romina Manarin, Jorge Barrios-Payán, Brenda Marquina-Castillo, Luis Tavernelli, Rogelio Hernández-Pando
Trypanosoma cruzi High Mobility Group B (TcHMGB) can act as an inflammatory mediator on mammalian cells
www.plosntds.org
www.plosntds.org
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(8)Tj
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0.83 0.64 0.02 0 k
(9)Tj
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(10)Tj
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(12)Tj
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1.7292 0 Td
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0.83 0.64 0.02 0 k
(13)Tj
0 g
(].)Tj
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0.01 Tc
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3.9911 0 Td
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(14)Tj
0 g
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(18)Tj
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(].)Tj
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1.0431 0 Td
[()75()-20()-13()4()16()6()7()-3()11( )6()]TJ
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[(\n)1()-9(\r)]TJ
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[()97(\n)]TJ
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(18)Tj
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5.7713 0 Td
[()97(\n)]TJ
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[()97(\n)]TJ
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3.8437 0 Td
[(\r)12()]TJ
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[()-13(\r)14()7()20()]TJ
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2.1146 0 Td
(and)Tj
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1.7745 0 Td
[(\r)12()]TJ
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()Tj
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(,)Tj
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()Tj
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(\r)Tj
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()Tj
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()Tj
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(\))Tj
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[()97(\n)]TJ
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(HMGB.)Tj
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4.5241 0 Td
(experimental)Tj
/F13 1 Tf
5.6239 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
1.0658 0 Td
[(\n)1()-9(\r)]TJ
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2.228 0 Td
(infection)Tj
3.9175 0 Td
(in)Tj
1.0658 0 Td
(BALB/c)Tj
3.4356 0 Td
(mice,)Tj
-33.3012 -1.2983 Td
(showed)Tj
3.3335 0 Td
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3.0047 0 Td
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2.3981 0 Td
(infection)Tj
3.9118 0 Td
(numerous)Tj
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(cardiomyocytes)Tj
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1.7688 0 Td
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2.0522 0 Td
(amastigotes,)Tj
5.2838 0 Td
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2.7155 0 Td
(showed)Tj
3.3392 0 Td
(strong)Tj
2.8517 0 Td
(TcHMGB)Tj
4.2973 0 Td
(immunostaining.)Tj
7.3303 0 Td
(TcHMGB)Tj
4.2973 0 Td
(immunos-)Tj
-32.1673 -1.2982 Td
(taining)Tj
3.1464 0 Td
(was)Tj
1.7404 0 Td
(also)Tj
1.8255 0 Td
(observed)Tj
3.9062 0 Td
(in)Tj
1.0601 0 Td
(fibrin)Tj
2.5569 0 Td
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3.1747 0 Td
(intravascular)Tj
5.5729 0 Td
(and)Tj
1.7745 0 Td
(attached)Tj
3.6963 0 Td
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1.0545 0 Td
(the)Tj
1.5137 0 Td
(endocardium)Tj
-31.0221 -1.2983 Td
(in)Tj
1.0601 0 Td
(coexistence)Tj
4.9379 0 Td
(with)Tj
2.058 0 Td
(numerous)Tj
4.4163 0 Td
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5.8734 0 Td
(cells)Tj
1.9955 0 Td
(that)Tj
1.8312 0 Td
(showed)Tj
3.3335 0 Td
(strong)Tj
2.8573 0 Td
(immunostaining)Tj
7.0923 0 Td
(to)Tj
-35.4555 -1.2983 Td
(TNF-)Tj
/F14 1 Tf
()Tj
/F8 1 Tf
(,)Tj
3.3221 0 Td
(IL-1)Tj
/F14 1 Tf
()Tj
/F8 1 Tf
2.4832 0 Td
(and)Tj
1.7688 0 Td
(IFN-)Tj
/F14 1 Tf
(\r)Tj
/F8 1 Tf
(.)Tj
2.9423 0 Td
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2.5398 0 Td
(immunohistochemistry)Tj
9.8986 0 Td
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3.0103 0 Td
(acute)Tj
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(experi-)Tj
-34.3556 -1.3039 Td
(mental)Tj
/F13 1 Tf
3.0727 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F13 1 Tf
1.0658 0 Td
[(\n)1()-9(\r)]TJ
/F8 1 Tf
2.228 0 Td
(infection)Tj
3.9175 0 Td
(showed)Tj
3.3335 0 Td
(high)Tj
2.0636 0 Td
(production)Tj
4.8586 0 Td
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1.0431 0 Td
(TcHMGB)Tj
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(amastigotes)Tj
5.0456 0 Td
(that)Tj
1.8312 0 Td
(appar-)Tj
-33.9588 -1.2983 Td
(ently)Tj
2.262 0 Td
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0.873 0 Td
(secreted)Tj
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1.7745 0 Td
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(cytokines.)Tj
-0.004 Tc
1.1962 -1.3039 Td
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0.01 Tc
2.5681 0 Td
(results)Tj
2.9084 0 Td
(suggest)Tj
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7.4999 0 0 6.6659 470.4944 648.3968 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
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10 0 0 10 476.2771 650.0976 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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(solutions)Tj
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(prepared)Tj
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 608.0314 cm
BT
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1 TL
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Q
q
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BT
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6.0095 0 Td
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0.7143 0 Td
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0.805 0 Td
(530)Tj
1.6441 0 Td
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3.9174 0 Td
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2.2507 0 Td
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1.7915 0 Td
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 458.022 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
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3.2362 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 441.0141 cm
BT
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1 TL
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2.2166 0 Td
(BALB/c)Tj
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[()6()]TJ
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[()12(\r)14()11(\r)14()7()12( )]TJ
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-35.8183 -1.2983 Td
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(\(i.p.\))Tj
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(saline)Tj
2.5002 0 Td
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-35.6766 -1.2983 Td
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2.982 0 Td
(\(r)Tj
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[()97(\n)]TJ
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(HMGB)Tj
4.8415 0 Td
(1)Tj
/F15 1 Tf
[()]TJ
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1.8879 0 Td
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(50)Tj
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[()]TJ
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1.4116 0 Td
(LPS)Tj
1.7858 0 Td
(1)Tj
/F15 1 Tf
[()]TJ
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(rTcHMGB)Tj
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[()]TJ
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(.)Tj
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1.0374 0 Td
[(\n)1()-9(\r)]TJ
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(.)Tj
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(\(acute\))Tj
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Q
q
1 j
1 J
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BT
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1 TL
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1 J
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BT
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1 TL
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-35.4838 -1.3039 Td
(10%)Tj
1.9275 0 Td
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(TNF)Tj
/F14 1 Tf
()Tj
/F8 1 Tf
(,)Tj
2.8913 0 Td
(IFN-)Tj
/F14 1 Tf
(\r)Tj
/F8 1 Tf
(,)Tj
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(IL-1)Tj
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(and)Tj
-34.9396 -1.2983 Td
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[()]TJ
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