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Jean-Yves Brossas, Julián Ernesto Nicolás Gulin, Margarita Maria Catalina Bisio, Manuel Chapelle, Carine Marinach-Patrice, Mallaury Bordessoules, George Palazon Ruiz, Jeremy Vion, Luc Paris, Jaime Altcheh, Dominique Mazier
Secretome analysis of Trypanosoma cruzi by proteomics studies
www.plosone.org
www.plosone.org
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[(\n)1()-9(\r)]TJ
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3.1068 0 Td
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 466.696 cm
BT
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1 TL
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(and)Tj
1.989 0 Td
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Q
q
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1 J
0 w
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BT
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1 TL
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1.0148 0 Td
[()-2()2()20(\n)7()11()11(\r)14()10()6()7(\n)1()-4()]TJ
5.6012 0 Td
[()6()8()10()18(\r)4()11()7()]TJ
/F8 1 Tf
(\))Tj
3.7814 0 Td
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2.1203 0 Td
(obtained)Tj
3.736 0 Td
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3.7303 0 Td
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1.4173 0.0567 Td
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[()]TJ
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[()]TJ
/F8 1 Tf
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4.4504 0 Td
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3.6227 0 Td
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2.7779 0 Td
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2.3471 0 Td
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(technologies\))Tj
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2.0012 0 Td
(streptomycin/penicillin)Tj
9.5924 0 Td
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(technologies\))Tj
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(5%)Tj
1.4457 0 Td
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6.6217 0 Td
(foetal)Tj
2.4094 0 Td
(bovine)Tj
2.9197 0 Td
(serum)Tj
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(\(FBS\))Tj
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(technologies\).)Tj
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0.9525 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 410.3433 365.0456 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 413.8582 366.6897 cm
BT
/F8 1 Tf
1 TL
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Q
q
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1 J
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Q
q
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BT
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3.9288 0 Td
(\(Paris,)Tj
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1.8255 0.051 Td
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4.5864 0 Td
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(Argentina.\))Tj
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(laboratory\))Tj
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1.1678 0 Td
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 242.5322 217.4173 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F8 1 Tf
1 TL
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(flasks)Tj
2.4037 0 Td
(with)Tj
2.0013 0 Td
(10)Tj
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Q
q
1 j
1 J
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7.4999 0 0 6.6659 301.8897 217.4173 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
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(4)Tj
ET
Q
q
1 j
1 J
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BT
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1 TL
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(per)Tj
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Q
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1 j
1 J
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7.4999 0 0 6.6659 354.5574 217.4173 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
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(2)Tj
ET
Q
q
1 j
1 J
0 w
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BT
/F8 1 Tf
1 TL
-0.004 Tc
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(,)Tj
0.4478 0 Td
(followed)Tj
3.6454 0 Td
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1.1848 0 Td
(3)Tj
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(days)Tj
1.9956 0 Td
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1.0148 0 Td
(incubation)Tj
4.5468 0 Td
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0.9524 0 Td
(37C)Tj
2.228 0 Td
(in)Tj
1.0375 0 Td
(5%)Tj
1.4513 0 Td
(CO)Tj
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Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 564.2645 211.3511 cm
BT
/F8 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 200.0125 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
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1.0318 0 Td
(air.)Tj
1.5193 0 Td
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(then)Tj
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1.9956 0 Td
[()]TJ
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1.0375 0 Td
[(\n)1()-9(\r)]TJ
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2.16 0 Td
(trypomastigotes)Tj
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(a)Tj
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(24)Tj
1.1678 0 Td
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(buffer)Tj
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(in)Tj
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(order)Tj
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(to)Tj
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5.5388 0 Td
(cells)Tj
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(free)Tj
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