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María A. Correa Deza, Mariana Grillo-Puertas, Susana Salva, Viviana A. Rapisarda, Carla L. Gerez, Graciela Font de Valdez
Inorganic salts and intracellular polyphosphate inclusions play a role in the thermotolerance of the immunobiotic Lactobacillus rhamnosus CRL 1505
www.plosone.org
www.plosone.org
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(shock\).)Tj
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 600.7748 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Effect)Tj
2.759 0 Td
(of)Tj
1.0441 0 Td
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ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 583.8236 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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1.7801 0 Td
(effect)Tj
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(20)Tj
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(harvested)Tj
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(15)Tj
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(min,)Tj
2.0806 0 Td
(4C\))Tj
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(and)Tj
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(inoculated)Tj
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(\(2%)Tj
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(v/v\))Tj
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(the)Tj
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(following)Tj
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(heat-shock)Tj
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(phosphate)Tj
4.337 0 Td
(buffer)Tj
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(after)Tj
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(heat)Tj
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(shock)Tj
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Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 490.2236 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Flow)Tj
2.3102 0 Td
(cytometry)Tj
4.5166 0 Td
(analysis)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 473.2157 cm
BT
/F7 1 Tf
1 TL
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[()]TJ
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2.33 0 Td
(Biosciences,)Tj
5.0286 0 Td
(USA\).)Tj
2.6816 0 Td
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(e.g.,)Tj
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(the)Tj
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(retention)Tj
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(thiazole)Tj
3.3336 0 Td
(orange)Tj
2.9707 0 Td
(\(TO\))Tj
2.2563 0 Td
(reveals)Tj
2.9367 0 Td
(themembrane)Tj
5.8167 0 Td
(integrity,)Tj
3.838 0 Td
(a)Tj
0.6577 0 Td
(parameter)Tj
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(of)Tj
1.0148 0 Td
(cell)Tj
1.559 0 Td
(viability;)Tj
3.6454 0 Td
(while)Tj
-33.3522 -1.2983 Td
(the)Tj
1.4683 0 Td
(accumulation)Tj
5.6749 0 Td
(of)Tj
1.0148 0 Td
(propidium)Tj
4.5468 0 Td
(iodine)Tj
2.7609 0 Td
(\(PI\))Tj
1.7971 0 Td
(indicates)Tj
3.7701 0 Td
(damaged)Tj
3.8551 0 Td
(plasma)Tj
3.0274 0 Td
(membranes)Tj
4.9322 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.075 0 Td
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-34.9226 -1.2983 Td
(cells)Tj
1.9218 0 Td
(were)Tj
2.1203 0 Td
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4.4391 0 Td
(in)Tj
1.0318 0 Td
(phosphate-buffered)Tj
8.0446 0 Td
(saline)Tj
2.5002 0 Td
(solution)Tj
3.4809 0 Td
(to)Tj
1.0261 0 Td
(approximately)Tj
5.9754 0 Td
(a)Tj
0.652 0 Td
(concentration)Tj
-31.1922 -1.3039 Td
(of)Tj
1.0147 0 Td
(1)Tj
0.6917 0 Td
(x)Tj
0.686 0 Td
(10)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 233.4614 412.6677 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(6)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 239.244 408.1889 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(cells)Tj
1.9162 0 Td
(ml)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 269.0645 412.6677 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(-1)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 277.4551 408.1889 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(incubated)Tj
4.1782 0 Td
(at)Tj
0.9524 0 Td
(room)Tj
2.4265 0 Td
(temperature)Tj
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(for)Tj
1.3833 0 Td
(5)Tj
0.6973 0 Td
(min)Tj
1.8538 0 Td
(with)Tj
1.9956 0 Td
(420)Tj
/F15 1 Tf
1.6498 0 Td
[()]TJ
/F7 1 Tf
(M)Tj
1.6214 0 Td
(for)Tj
1.3833 0 Td
(TO)Tj
1.576 0 Td
(and)Tj
-34.3216 -1.2982 Td
(48)Tj
/F15 1 Tf
1.1679 0 Td
[()]TJ
/F7 1 Tf
(M)Tj
1.6214 0 Td
(for)Tj
1.389 0 Td
(PI.)Tj
1.3436 0 Td
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1.8992 0 Td
(untreated)Tj
4.0761 0 Td
(cells)Tj
1.9219 0 Td
(\(37C)Tj
2.5625 0 Td
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(5)Tj
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(\(60C)Tj
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(5)Tj
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(min)Tj
-34.8092 -1.2983 Td
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1.0148 0 Td
[()]TJ
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(.)Tj
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0.9694 0 Td
[(\r)8()6()16()18()6()7(\n)10( )-4(\n)]TJ
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4.5353 0 Td
(CRL)Tj
2.041 0 Td
(1505)Tj
2.1203 0 Td
(in)Tj
1.0374 0 Td
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3.3165 0 Td
(and)Tj
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-33.3578 -1.3039 Td
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(respectively.)Tj
5.1079 0 Td
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(analyzed)Tj
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2.0012 0 Td
(a)Tj
0.652 0 Td
(FACS)Tj
2.5738 0 Td
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3.2315 0 Td
(flow)Tj
1.9502 0 Td
(cytometer)Tj
4.235 0 Td
(\(BD)Tj
/F15 1 Tf
[()]TJ
/F7 1 Tf
2.3357 0 Td
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-35.1153 -1.2983 Td
(sciences,)Tj
3.6737 0 Td
(USA\))Tj
2.4605 0 Td
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(488)Tj
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1.7802 0 Td
(BD)Tj
1.5363 0 Td
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2.058 0 Td
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(sheath)Tj
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2.5965 0 Td
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1.6555 0 Td
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3.0217 0 Td
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3.3959 0 Td
(7.6.1.)Tj
2.3187 0 Td
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(injured,)Tj
3.3676 0 Td
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(dead)Tj
2.1316 0 Td
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Q
q
1 j
1 J
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11.9999 0 0 11.9999 200.0125 288.6236 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
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6.0992 0 Td
(electron)Tj
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ET
Q
q
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1 J
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10 0 0 10 200.0125 271.6157 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
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(subjected)Tj
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(\(60C,)Tj
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(5)Tj
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5.9924 0 Td
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6.1285 0 Td
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(22)Tj
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2.262 0 Td
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(tetroxide)Tj
3.804 0 Td
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2.2564 0 Td
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0.9524 0 Td
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1.7518 0 Td
(overnight.)Tj
4.3086 0 Td
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4.7395 0 Td
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1.0318 0 Td
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2.4264 0 Td
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1.7291 0 Td
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2.3981 0 Td
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2.1997 0 Td
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4.8812 0 Td
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1.8539 0 Td
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1.7348 0 Td
(uranyl)Tj
2.8006 0 Td
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([)Tj
0.83 0.64 0.02 0 k
(23)Tj
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0.6577 0 Td
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2.2337 0 Td
(EM109)Tj
3.1067 0 Td
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3.4639 0 Td
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4.8416 0 Td
(\(Carl)Tj
2.2733 0 Td
(Zeiss)Tj
2.2337 0 Td
(NTS)Tj
2.041 0 Td
(GmbH,)Tj
3.2541 0 Td
(Oberkochen,)Tj
5.4198 0 Td
(Ger-)Tj
-35.6482 -1.3039 Td
(many\).)Tj
3.0387 0 Td
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3.6113 0 Td
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5.2837 0 Td
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1.0147 0 Td
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0.7937 0 Td
(50,000)Tj
2.8233 0 Td
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[()]TJ
/F7 1 Tf
2.2961 0 Td
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ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 139.011 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
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6.7654 0 Td
(of)Tj
1.0488 0 Td
(polyP)Tj
2.6882 0 Td
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Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 122.0031 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
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2.5058 0 Td
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