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Julia A. Loos, Valeria A. Dávila, Christian R. Rodrígues, Romina Petrigh, Jorge A. Zoppi, Fernando A. Crocenzi, Andrea C. Cumino
Metformin exhibits preventive and therapeutic efficacy against experimental cystic echinococcosis
www.plosntds.org
www.plosntds.org
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0 -1.5023 Td
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1.6271 0 Td
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1.0148 0 Td
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Q
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BT
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Q
q
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3.1975 0 Td
(\(SENASA\))Tj
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3.685 0 Td
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3.1351 0 Td
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5.6012 0 Td
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5.6069 0 Td
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(the)Tj
1.4683 0 Td
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3.1351 0 Td
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2.4094 0 Td
(and)Tj
1.7291 0 Td
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3.2655 0 Td
(Sciences,)Tj
3.7928 0 Td
(Mar)Tj
1.9162 0 Td
(del)Tj
1.4173 0 Td
(Plata)Tj
2.194 0 Td
(Uni-)Tj
-35.3591 -1.2982 Td
(versity)Tj
2.8516 0 Td
(\(permit)Tj
3.2541 0 Td
(number:)Tj
3.6454 0 Td
(2555-08-15\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 217.0204 cm
BT
/F5 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(In)Tj
1.0441 0 Td
(vitro)Tj
/F0 1 Tf
2.0882 0 Td
(drug)Tj
2.1921 0 Td
(testing)Tj
3.1323 0 Td
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3.3024 0 Td
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1.3181 0 Td
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2.5701 0 Td
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1.0441 0 Td
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(.)Tj
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1.1622 0 Td
(granulosus)Tj
ET
Q
q
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1 J
0 w
10 0 0 10 200.0125 200.0125 cm
BT
/F6 1 Tf
1 TL
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5.6295 0 Td
(were)Tj
2.126 0 Td
(removed)Tj
3.7474 0 Td
(aseptically)Tj
4.3256 0 Td
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2.2054 0 Td
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3.2541 0 Td
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(of)Tj
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3.4186 0 Td
(cattle)Tj
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(slaughtered)Tj
4.8246 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
-36.0507 -1.3039 Td
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3.3391 0 Td
(abattoir)Tj
3.3392 0 Td
(\(official)Tj
3.2769 0 Td
(number:)Tj
3.6453 0 Td
(3879\))Tj
2.4605 0 Td
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3.084 0 Td
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(the)Tj
1.474 0 Td
(Southeast)Tj
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3.1691 0 Td
(Aires,)Tj
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(Argentina.)Tj
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(and)Tj
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(morphologically)Tj
6.7635 0 Td
(intact)Tj
2.4888 0 Td
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5.5899 0 Td
(\()Tj
/F7 1 Tf
[()]TJ
/F6 1 Tf
1.0885 0 Td
(=)Tj
0.7993 0 Td
(3,000\))Tj
2.6872 0 Td
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(199)Tj
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(\(Gibco\))Tj
3.3165 0 Td
(supplemented)Tj
5.8563 0 Td
(with)Tj
1.9956 0 Td
(glucose)Tj
3.1748 0 Td
(\(4)Tj
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(mg/ml\))Tj
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(and)Tj
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(antibiotics)Tj
4.3993 0 Td
(\(penicillin,)Tj
4.5411 0 Td
(streptomycin)Tj
5.5105 0 Td
(and)Tj
-34.8035 -1.2983 Td
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4.7168 0 Td
(100)Tj
/F12 1 Tf
1.6441 0 Td
[()]TJ
/F6 1 Tf
(g/ml\))Tj
2.9253 0 Td
(in)Tj
1.0375 0 Td
(24-well)Tj
3.1237 0 Td
(culture)Tj
3.0387 0 Td
(plates)Tj
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2.6192 0 Td
(normal)Tj
3.1464 0 Td
(atmospheric)Tj
5.1704 0 Td
(conditions)Tj
4.473 0 Td
(as)Tj
1.0148 0 Td
(we)Tj
-35.4101 -1.3039 Td
(described)Tj
4.0535 0 Td
(in)Tj
1.0318 0 Td
(detail)Tj
2.4207 0 Td
(previously)Tj
4.3484 0 Td
([)Tj
0.83 0.64 0.02 0 k
(15)Tj
0 g
(].)Tj
2.0749 0 Td
(Moreover,)Tj
4.3994 0 Td
(from10)Tj
3.1577 0 Td
(to)Tj
1.0205 0 Td
(20)Tj
/F7 1 Tf
1.1735 0 Td
[()]TJ
/F6 1 Tf
(.)Tj
/F7 1 Tf
0.9922 0 Td
[( )-8(\n)20()16()18()4()4()7()10()-4()]TJ
/F6 1 Tf
4.4276 0 Td
(murine)Tj
3.1748 0 Td
(cysts)Tj
2.1203 0 Td
(per)Tj
1.5307 0 Td
(rep-)Tj
-35.926 -1.2982 Td
(lica)Tj
1.5873 0 Td
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2.126 0 Td
(incubated)Tj
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1.0318 0 Td
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3.7984 0 Td
(tubes)Tj
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(for)Tj
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(toscoleces)Tj
4.1952 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
/F7 1 Tf
2.0749 0 Td
[()39()]TJ
1.0772 0 Td
[(\r)-13()14()7(\n)20()]TJ
/F6 1 Tf
2.0466 0 Td
(protoscolex)Tj
4.8472 0 Td
(and)Tj
1.7291 0 Td
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5.1591 0 Td
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(1)Tj
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(2.5)Tj
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[()]TJ
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(\(equivalent)Tj
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(0.84)Tj
/F12 1 Tf
1.8709 0 Td
[()]TJ
/F6 1 Tf
(g/ml\),)Tj
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(and)Tj
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(the)Tj
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(2.5)Tj
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[()]TJ
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(7)Tj
0.6973 0 Td
(\(metacestodes\))Tj
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(and)Tj
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(27)Tj
1.1679 0 Td
(\(protoscoleces\))Tj
6.2702 0 Td
(days)Tj
1.9955 0 Td
([)Tj
0.83 0.64 0.02 0 k
(16)Tj
0 g
(].)Tj
2.0807 0 Td
(Parasites)Tj
3.7303 0 Td
(incubated)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 183.2882 23.6976 re
W* n
q
183.2315 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 366.5763 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Improvem)Tj
4.3795 0 Td
(ent)Tj
1.5874 0 Td
(in)Tj
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