Artículo
Purification of the lipase B of Candida antarctica from a commercial enzymatic extract
Fecha de publicación:
01/2014
Editorial:
Bentham Science Publishers
Revista:
Current Catalysis
ISSN:
2211-5447
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.
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Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CINDECA)
Articulos de CENTRO DE INV EN CS.APLICADAS "DR.JORGE J.RONCO"
Articulos de CENTRO DE INV EN CS.APLICADAS "DR.JORGE J.RONCO"
Citación
Llerena Suster, Carlos Rafael; Briand, Laura Estefania; Morcelle del Valle, Susana Raquel; Purification of the lipase B of Candida antarctica from a commercial enzymatic extract; Bentham Science Publishers; Current Catalysis; 3; 2; 1-2014; 155-160
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