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dc.contributor.author Gutiérrez Cacciabue, Dolores
dc.contributor.author Cid, Alicia Graciela
dc.contributor.author Rajal, Verónica Beatriz
dc.date.available 2018-10-10T20:44:01Z
dc.date.issued 2016-01
dc.identifier.citation Gutiérrez Cacciabue, Dolores; Cid, Alicia Graciela; Rajal, Verónica Beatriz; How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles; Elsevier Science; Science of the Total Environment; 539; 1-2016; 494-502
dc.identifier.issn 0048-9697
dc.identifier.uri http://hdl.handle.net/11336/62151
dc.description.abstract In this work, sunlight inactivation of two indicator bacteria in freshwater, with and without solid particles, was studied and the persistence of culturable cells and total DNA was compared. Environmental water was used to prepare two matrices, with and without solid particles, which were spiked with Escherichia coli and Enterococcus faecalis. These matrices were used to prepare microcosm bags that were placed in two containers: one exposed to sunlight and the other in the dark. During one month, samples were removed from each container and detection was done by membrane filter technique and real-time PCR. Kinetic parameters were calculated to assess sunlight effect. Indicator bacteria without solid particles exposed to sunlight suffered an immediate decay (<4h) compared with the ones which were shielded from them. In addition, the survival of both bacteria with solid particles varied depending on the situation analyzed (T99 from 3 up to 60days), being always culturable E. coli more persistent than E. faecalis. On the other side, E. faecalis DNA persisted much longer than culturable cells (T99>40h in the dark with particles). In this case active cells were more prone to sunlight than total DNA and the protective effect of solid particles was also observed. Results highlight that the effects caused by the parameters which describe the behavior of culturable microorganisms and total DNA in water are different and must be included in simulation models but without forgetting that these parameters will also depend on bacterial properties, sensitizers, composition, type, and uses of the aquatic environment under assessment.
dc.format application/pdf
dc.language.iso eng
dc.publisher Elsevier Science
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BACTERIAL INDICATORS
dc.subject CULTURE-BASED METHODS
dc.subject ENVIRONMENTAL WATERS
dc.subject QPCR
dc.subject SOLID PARTICLES
dc.subject SUNLIGHT INACTIVATION
dc.subject.classification Ingeniería Medioambiental y Geológica, Geotécnicas
dc.subject.classification Ingeniería del Medio Ambiente
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title How long can culturable bacteria and total DNA persist in environmental waters? The role of sunlight and solid particles
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-09-27T15:35:54Z
dc.journal.volume 539
dc.journal.pagination 494-502
dc.journal.pais Países Bajos
dc.journal.ciudad Amsterdam
dc.description.fil Fil: Gutiérrez Cacciabue, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
dc.description.fil Fil: Cid, Alicia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
dc.description.fil Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Nanyang Technological University; Singapur
dc.journal.title Science of the Total Environment
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.scitotenv.2015.07.138
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0048969715304800
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)