Artículo
Antiviral activity of natural and synthetic β-carbolines against dengue virus
Quintana, Verónica Mara
; Piccini, Luana Érica
; Panozzo Zénere, Juan David; Damonte, Elsa Beatriz
; Ponce, María Alejandra; Castilla, Viviana
Fecha de publicación:
10/2016
Editorial:
Elsevier Science
Revista:
Antiviral Research
ISSN:
0166-3542
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Dengue virus (DENV) is the most prevalent mosquito borne viral pathogen worldwide. In this work we first evaluated the antiviral activity of natural and synthetic β-carbolines against DENV-2 multiplication in cell cultures. We determined that the natural β-carboline harmol and a synthetic harmine derivative, 9N-methylharmine, exhibit inhibitory effect on DENV-2 production without virucidal activity. The active compounds were inhibitory of all DENV serotypes, being DENV-2 the more susceptible to their antiviral action. The mode of action of 9N-methylharmine against DENV-2 was further explored. We determined that the derivative neither affects viral adsorption-internalization events nor viral RNA synthesis. The quantification of intracellular and extracellular viral genomes and infectious virus particles indicated that 9N-methylharmine would impair the maturation and release of virus particles to the extracellular medium affecting the spreading of the infection. Furthermore, we also determined that 9N-methylharmine antiviral activity is not related to the ability of the compound to downregulate p38 MAPK phosphorylation.
Palabras clave:
Beta-Carbolines
,
Dengue Virus
,
Antiviral Activity
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Articulos(IQUIBICEN)
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Articulos de INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CS. EXACTAS Y NATURALES
Citación
Quintana, Verónica Mara; Piccini, Luana Érica; Panozzo Zénere, Juan David; Damonte, Elsa Beatriz; Ponce, María Alejandra; et al.; Antiviral activity of natural and synthetic β-carbolines against dengue virus; Elsevier Science; Antiviral Research; 134; 10-2016; 26-33
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