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dc.contributor.author Hill, Benjamin L.
dc.contributor.author Figueroa, Carlos Maria
dc.contributor.author Asención Diez, Matías Damián
dc.contributor.author Lunn, John E.
dc.contributor.author Iglesias, Alberto Alvaro
dc.contributor.author Ballicora, Miguel A.
dc.date.available 2018-05-16T19:40:09Z
dc.date.issued 2017-06
dc.identifier.citation Hill, Benjamin L.; Figueroa, Carlos Maria; Asención Diez, Matías Damián; Lunn, John E.; Iglesias, Alberto Alvaro; et al.; On the stability of nucleoside diphosphate glucose metabolites: implications for studies of plant carbohydrate metabolism; Oxford University Press; Journal of Experimental Botany; 68; 13; 6-2017; 3331-3337
dc.identifier.issn 0022-0957
dc.identifier.uri http://hdl.handle.net/11336/45427
dc.description.abstract Nucleoside diphosphate sugars (NDP-sugars) are the substrates for biosynthesis of oligo- and polysaccharides, such as starch and cellulose, and are also required for biosynthesis of nucleotides, ascorbic acid, several cofactors, glycoproteins and many secondary metabolites. A controversial study that questions the generally accepted pathway of ADP-glucose and starch synthesis in plants is based, in part, on claims that NDP-sugars are unstable at alkaline pH in the presence of Mg2+ and that this instability can lead to unreliable results from in vitro assays of enzyme activities. If substantiated, this claim would have far-reaching implications for many published studies that report on the activities of NDP-sugar metabolizing enzymes. To resolve this controversy, we investigated the stability of UDP- and ADP-glucose using biophysical, namely nuclear magnetic resonance (NMR), and highly specific enzymatic methods. Results obtained with both techniques indicate that NDP-sugars are not as unstable as previously suggested. Moreover, their calculated in vitro half-lives are significantly higher than estimates of their in planta turnover times. This indicates that the physico-chemical stability of NDP-sugars has little impact on their concentrations in vivo and that NDP-sugar levels are determined primarily by the relative rates of enzymatic synthesis and consumption. Our results refute one of the main arguments for the controversial pathway of starch synthesis from imported ADP-glucose produced by sucrose synthase in the cytosol.
dc.format application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ADP-GLUCOSE
dc.subject NMR
dc.subject PLASTID
dc.subject STARCH
dc.subject SUCROSE
dc.subject UDP-GLUCOSE
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title On the stability of nucleoside diphosphate glucose metabolites: implications for studies of plant carbohydrate metabolism
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-05-11T21:17:46Z
dc.journal.volume 68
dc.journal.number 13
dc.journal.pagination 3331-3337
dc.journal.pais Reino Unido
dc.journal.ciudad Oxford
dc.description.fil Fil: Hill, Benjamin L.. Loyola University Chicago; Estados Unidos
dc.description.fil Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Asención Diez, Matías Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Lunn, John E.. Max Planck Institute Of Molecular Plant Physiology; Alemania
dc.description.fil Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil Fil: Ballicora, Miguel A.. Loyola University Chicago; Estados Unidos
dc.journal.title Journal of Experimental Botany
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/68/13/3331/3867363
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/erx190
dc.conicet.fuente individual


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