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dc.contributor.author Trangoni, Marcos D.
dc.contributor.author Gioffré, Andrea Karina
dc.contributor.author Cravero, Silvio Lorenzo Pedro
dc.date.available 2018-04-16T16:23:14Z
dc.date.issued 2017-06
dc.identifier.citation Trangoni, Marcos D.; Gioffré, Andrea Karina; Cravero, Silvio Lorenzo Pedro; A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis; Humana Press; Methods in Molecular Biology; 1616; 6-2017; 221-229
dc.identifier.issn 1064-3745
dc.identifier.uri http://hdl.handle.net/11336/42124
dc.description.abstract LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine.
dc.format application/pdf
dc.language.iso eng
dc.publisher Humana Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BRUCELLA
dc.subject BRUCELLOSIS
dc.subject ISOTHERMAL AMPLIFICATION
dc.subject LAMP
dc.subject MOLECULAR DETECTION
dc.subject MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS
dc.subject PARATUBERCULOSIS
dc.subject.classification Otras Ciencias Biológicas
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-04-05T13:43:40Z
dc.journal.volume 1616
dc.journal.pagination 221-229
dc.journal.pais Estados Unidos
dc.journal.ciudad Clifton
dc.description.fil Fil: Trangoni, Marcos D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil Fil: Gioffré, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.description.fil Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
dc.journal.title Methods in Molecular Biology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/978-1-4939-7037-7_14
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/protocol/10.1007%2F978-1-4939-7037-7_14
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)