Artículo
Preferential transfer of the complete glycan is determined by the oligosaccharyltransferase complex and not by the catalytic subunit
Fecha de publicación:
10/2006
Editorial:
National Academy of Sciences
Revista:
Proceedings of the National Academy of Sciences of The United States of America
ISSN:
0027-8424
e-ISSN:
1091-6490
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Most eukaryotic cells show a strong preference for the transfer in vivo and in vitro of the largest dolichol-P-P-linked glycan (Glc3Man 9GlcNAc2) to protein chains over that of biosynthetic intermediates that lack the full complement of glucose units. The oligosaccharyltransferase (OST) is a multimeric complex containing eight different proteins, one of which (Stt3p) is the catalytic subunit. Trypanosomatid protozoa lack an OST complex and express only this last protein. Contrary to the OST complex from most eukaryotic cells, the Stt3p subunit of these parasites transfers in cell-free assays glycans with Man 7-9GlcNAc2 and Glc1-3Man9GlcNAc 2 compositions at the same rate. We have replaced Saccharomyces cerevisiae Stt3p by the Trypanosoma cruzi homologue and found that the complex that is formed preferentially transfers the complete glycan both in vivo and in vitro. Thus, preference for Glc3Man9GlcNAc2 is a feature that is determined by the complex and not by the catalytic subunit.
Palabras clave:
N-GLYCOSYLATION
,
SACCHAROMYCES CEREVISIAE
,
TRYPANOSOMA CRUZI
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Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(OCA CIUDAD UNIVERSITARIA)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Citación
Castro, Olga Alejandra; Movsichoff, Federico; Parodi, Armando José A.; Preferential transfer of the complete glycan is determined by the oligosaccharyltransferase complex and not by the catalytic subunit; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 103; 40; 10-2006; 14756-14760
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