Artículo
Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - Unambiguous assignment of product ions by tandem mass spectrometry
Qi, Yulin; Rosso, Lorena Vanesa
; Sedan, Daniela Yazmine
; Giannuzzi, Leda
; Andrinolo, Dario
; Volmer, Dietrich A.
Fecha de publicación:
01/2015
Editorial:
John Wiley & Sons Ltd
Revista:
Rapid Communications in Mass Spectrometry
ISSN:
0951-4198
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
RATIONALE High-resolution mass spectrometry was applied to the study of a Microcystis aeruginosa strain previously reported as a [D-Leu1]MC-LR producer. Detailed analysis revealed new microcystin (MC) variants produced from the strain, and seven of these were previously unreported variants. This work shows the importance of mass accuracy for the identification of unknown MCs. METHODS The M. aeruginosa strain was isolated from a bloom sample collected from Argentina and acclimated to lab conditions. The MC variants in the strain were separated by UV/Vis detection-guided high-performance liquid chromatography, and their structures were unambiguous determined by tandem mass spectrometry (MS/MS). RESULTS A simple strategy was developed for quickly locating the low-abundance MC precursors from complex samples. MS/MS anlysis revealed ten MC variants produced from the strain, of which seven have never been reported before. CONCLUSIONS This work shows the interference of isobarics and isomers in the study of unknown MCs, and, therefore, high mass accuracy is important to avoid false assignments. Moreover, the peak list provided here (30-50 fragments unambiguously assigned for ten MCs) can be used as a reference for the discovery of MCs from environmental samples.
Palabras clave:
Ne Microcystins
,
Ms-Ms Detection
,
Microcystin Aeruginosa Strain
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Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CIDCA)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Articulos de CENTRO DE INV EN CRIOTECNOLOGIA DE ALIMENTOS (I)
Citación
Qi, Yulin; Rosso, Lorena Vanesa; Sedan, Daniela Yazmine; Giannuzzi, Leda; Andrinolo, Dario; et al.; Seven new microcystin variants discovered from a native Microcystis aeruginosa strain - Unambiguous assignment of product ions by tandem mass spectrometry; John Wiley & Sons Ltd; Rapid Communications in Mass Spectrometry ; 29; 2; 1-2015; 220-224
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