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dc.contributor.author Garbarino Azúa, Darío Jose
dc.contributor.author Saucedo, Lucia
dc.contributor.author Giordana, S.
dc.contributor.author Magri, M.L.
dc.contributor.author Buffone, Mariano Gabriel
dc.contributor.author Neuspiller, F.
dc.contributor.author Vazquez, Monica Hebe
dc.contributor.author Marin Briggiler, Clara Isabel
dc.date.available 2018-02-12T15:51:25Z
dc.date.issued 2017-09
dc.identifier.citation Garbarino Azúa, Darío Jose; Saucedo, Lucia; Giordana, S.; Magri, M.L.; Buffone, Mariano Gabriel; et al.; Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery; Wiley-liss, Div John Wiley & Sons Inc; Andrology; 5; 5; 9-2017; 990-998
dc.identifier.issn 2047-2919
dc.identifier.uri http://hdl.handle.net/11336/36420
dc.description.abstract Fibroblast growth factors (FGFs) and their receptors (FGFRs) regulate several functions of somatic cells. In a previous work, we reported FGFR expression in human spermatozoa and their involvement in motility. This study aimed to evaluate the presence and localization of fibroblast growth factor 2 (FGF2) in human spermatozoa, to determine the relationship of FGF2 levels with conventional semen parameters and to assess the effect of recombinant FGF2 (rFGF2) on sperm recovery in a selection procedure. Western immunoblotting analysis using an antibody against FGF2 revealed an 18-kDa band in sperm protein extracts. The protein was immunolocalized in the sperm flagellum and acrosomal region, as well as in all germ cells. Sperm FGF2 levels, assessed by flow cytometry, showed a positive (p < 0.05) correlation with sperm concentration, motility, total sperm number and total motile cells per ejaculate. Moreover, samples with abnormal motility depicted diminished (p < 0.01) FGF2 levels compared to those with normal motility. Spermatozoa exposed to rFGF2 bound the protein, exhibited higher (p < 0.05) total and motile sperm recoveries, and increased (p < 0.01) kinematic parameters after the swim-up. Findings herein presented lead to consider sperm FGF2 level as a potential marker of sperm quality, and rFGF2 as a supplement for improving sperm recovery in selection techniques.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley-liss, Div John Wiley & Sons Inc
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject FGF2
dc.subject FIBROBLAST GROWTH FACTOR 2
dc.subject MOTILITY
dc.subject SPERMATOZOA
dc.subject SWIM-UP
dc.subject.classification Biología Reproductiva
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility: the use of recombinant FGF2 to improve motile sperm recovery
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2018-02-05T13:42:47Z
dc.identifier.eissn 2047-2927
dc.journal.volume 5
dc.journal.number 5
dc.journal.pagination 990-998
dc.journal.pais Reino Unido
dc.description.fil Fil: Garbarino Azúa, Darío Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Saucedo, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Giordana, S.. Instituto Valenciano de Infertilidad ; Argentina
dc.description.fil Fil: Magri, M.L.. Instituto Valenciano de Infertilidad ; Argentina
dc.description.fil Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Neuspiller, F.. Instituto Valenciano de Infertilidad ; Argentina
dc.description.fil Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Marin Briggiler, Clara Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title Andrology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/andr.12398/abstract
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/andr.1239
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/28732140
dc.conicet.fuente Elsevier


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)