Artículo
Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability
Fecha de publicación:
01/2014
Editorial:
Royal Society of Chemistry
Revista:
Analyst
ISSN:
0003-2654
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The aims of this work were to efficiently immobilize organophosphate degrading enzyme, His6-OPH, on hierarchical mesoporoustitania thin films and further, to use this biocatalyst films as efficient tools in the detection/detoxofication of pesticides, paraoxon in our case. His6-OPH was immobilized on mesoporous thin films with uniform (9nm) and bimodal (13-38nm) pore size distribution, through covalent attachment and physical adsorption. The biocatalyst films show good activity, and enhanced stability with respect to the free enzyme at extreme conditions of pH and temperature, especially around neutral pH and room temperature. In addition, they can be easily separated from the reaction media and reused multiple times without significant loss in activity.
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Francica, Cynthia; Bellino, Martin Gonzalo; Soler Illia, Galo Juan de Avila Arturo; Lobnik, A.; Mesoporous titania thin films as efficient enzyme carriers for paraoxon determination/detoxification: effects of enzyme binding and pore hierarchy in biocatalyst activity and reusability; Royal Society of Chemistry; Analyst; 139; 1-2014; 3127-3136
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