Artículo
The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation
Shayo, Carina Claudia
; Lemos Legnazzi, Bibiana; Monczor, Federico
; Fernández, Natalia Brenda
; Riveiro, María Eugenia; Baldi, Alberto
; Davio, Carlos Alberto
Fecha de publicación:
12/2004
Editorial:
Elsevier Inc
Revista:
Biochemical and Biophysical Research Communications
ISSN:
0006-291X
e-ISSN:
1090-2104
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The regulation of the cAMP signaling is intimately involved in several cellular processes, including cell differentiation. Here, we provide strong evidence supporting that the time-course of cAMP signal is critical for leukemia U-937 cell differentiation. Three stimulating-cAMP agents were used to analyze the correlation between cAMP time-course and cell differentiation. All three agents denoted similar cAMP maximal responses in dose-response experiments. The kinetic of desensitization showed differential characteristics, while H2 receptor desensitized homologously without affecting PGE2 or forskolin effect, PGE2 response showed mixed desensitization characterized by a homologous initial phase followed by a heterologous phase. Regarding forskolin, long-term stimuli attenuated PGE2 and H2 agonist response without affecting adenylyl cyclase activity. In the absence of phosphodiesterase inhibitors, the three agents induced similar maximal cAMP levels after 5 min, but only that induced by the H2 agonist returned to basal levels. Consistent with this observation, H2 agonist was not able to induce U-937 cell maturation in contrast to PGE2 and forskolin, supporting the importance of time-course signaling in the determination of cell behavior.
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Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(ININFA)
Articulos de INST.DE INVEST.FARMACOLOGICAS (I)
Articulos de INST.DE INVEST.FARMACOLOGICAS (I)
Citación
Shayo, Carina Claudia; Lemos Legnazzi, Bibiana; Monczor, Federico; Fernández, Natalia Brenda; Riveiro, María Eugenia; et al.; The time-course of cyclic AMP signaling is critical for leukemia U-937 cell differentiation; Elsevier Inc; Biochemical and Biophysical Research Communications; 314; 3; 12-2004; 798-804
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