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dc.contributor.author Silva, I. T.
dc.contributor.author Teixeira, Marina R.
dc.contributor.author Lang, Karen L.
dc.contributor.author Guimarães, Tatiana da R.
dc.contributor.author Dudek, Sabine E.
dc.contributor.author Duran, Fernando Javier
dc.contributor.author Ludwig, Stephan
dc.contributor.author Caro, Miguel S. B.
dc.contributor.author Schenkel, Eloir P.
dc.contributor.author Simoes, Cláudia M. O.
dc.date.available 2017-10-23T18:08:28Z
dc.date.issued 2013-05
dc.identifier.citation Silva, I. T.; Teixeira, Marina R.; Lang, Karen L.; Guimarães, Tatiana da R.; Dudek, Sabine E.; et al.; Proliferative Inhibition and Apoptotic Mechanism on Human Non-small-cell Lung Cancer (A549 Cells) of a Novel Cucurbitacin from Wilbrandia ebracteata Cogn; Science Alert; International Journal of Cancer Research; 9; 2; 5-2013; 54-68
dc.identifier.issn 1811-9727
dc.identifier.uri http://hdl.handle.net/11336/26914
dc.description.abstract Cancer represents a major public health problem from all over the world and the lung carcinoma is the leading cause of cancer death. In this sense, the aim of the present study was to examine the cytotoxic effects of a novel Cucurbitacin (Cuc1) isolated from Wilbrandia ebracteata on human non-small-cell lung cancer (A549). In order to achieve this aim, the cell proliferation was measured by MTT assay and actin cytoskeleton was stained by rhodamine-phalloidin, whereas, the cell cycle distribution and apoptosis induction were quantified using flow cytometry. The signal transduction profiling of Cuc1 treated cells, as well as the levels of apoptotic proteins were analyzed by Western blotting. Cuc1 significantly inhibited cell growth showing IC50 values of 13.5±1.8 and 3.8±0.4 μM for 48 and 72 h of treatment, respectively. Additionally, Cuc1 arrested cell cycle at G2/M phase, disrupted the actin dynamics and induced apoptosis since the amount of apoptotic cells increased from 5.18±0.585% in the untreated cells to 73.82±0.545% in the treated cells. Detailed analysis on the mechanism of action revealed that Cuc1 inhibited the phosphorylation of Protein Kinase B (PKB/AKT) and Signal Transducer and Activator of Transcription (STAT3) signaling pathways, down-regulating the expression of Bcl-2 and consequently inducing cytochrome c release from the mitochondria to the cytosol. These results suggest that the Cuc1 could be a potential candidate for cancer chemotherapy agent.
dc.format application/pdf
dc.language.iso eng
dc.publisher Science Alert
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CUCURBITACINS
dc.subject LUNG CANCER
dc.subject A549 CELL
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Proliferative Inhibition and Apoptotic Mechanism on Human Non-small-cell Lung Cancer (A549 Cells) of a Novel Cucurbitacin from Wilbrandia ebracteata Cogn
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-06-22T17:36:37Z
dc.identifier.eissn 1811-9735
dc.journal.volume 9
dc.journal.number 2
dc.journal.pagination 54-68
dc.journal.pais Emiratos Árabes Unidos
dc.description.fil Fil: Silva, I. T.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Teixeira, Marina R.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Lang, Karen L.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Guimarães, Tatiana da R.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Dudek, Sabine E.. University of Münster; Alemania
dc.description.fil Fil: Duran, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
dc.description.fil Fil: Ludwig, Stephan. University of Münster; Alemania
dc.description.fil Fil: Caro, Miguel S. B.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Schenkel, Eloir P.. Universidade Federal de Santa Catarina; Brasil
dc.description.fil Fil: Simoes, Cláudia M. O.. Universidade Federal de Santa Catarina; Brasil
dc.journal.title International Journal of Cancer Research
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3923/ijcr.2013.54.68
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.scialert.net/abstract/?doi=ijcr.2013.54.68


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)