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dc.contributor.author Irusta, Griselda
dc.contributor.author Murphy, M. J.
dc.contributor.author Perez, W. D.
dc.contributor.author Hennebold J.D.
dc.date.available 2017-10-10T15:26:52Z
dc.date.issued 2007
dc.identifier.citation Irusta, Griselda; Murphy, M. J.; Perez, W. D.; Hennebold J.D.; Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum; Oxford University Press; Molecular Human Reproduction; 13; 8; -1-2007; 541-548
dc.identifier.issn 1360-9947
dc.identifier.uri http://hdl.handle.net/11336/26334
dc.description.abstract Epoxyeicosatrienoic acids (EpETrEs), produced from arachidonic acid via cytochrome P450 (CYP) epoxygenases, regulate inflammation, angiogenesis, cellular proliferation, ion transport and steroidogenesis. EpETrE actions are regulated through their metabolism to diols (dihydroxyeicosatrienoic acids; DiHETrE) via the enzyme soluble epoxide hydrolase (EPHX2). We set out to determine, therefore, whether EpETrE generating (epoxygenases CYP2C8, 2C9, 2C19, 2J2, 1A2 and 3A4) and metabolizing (EPHX2) enzymes are expressed in the primate corpus luteum (CL). CL were isolated from rhesus macaques during the early (day 3-5 post-LH surge), mid (day 6-8), mid-late (day 10-12), late (day 14-16) and very-late (day 17-19: menses) luteal phase of natural menstrual cycles. EPHX2 mRNA levels peaked in mid-late CL (5-fold when compared with early CL, P<0.05) and remained elevated in the late CL. Ablation of pituitary LH secretion and luteal steroid synthesis significantly reduced (P<0.05) EPHX2 mRNA levels in the mid-late CL, with progestin replacement being insufficient to restore its level of expression to control values. EPHX2 protein was localized to large and small luteal cells, as well as vascular endothelial cells. The EpETrE-generating CYP epoxygenase 2J2, 2C9 and 3A4 genes were also expressed in the macaque CL. While CYP2J2 mRNA levels did not significantly change through the luteal phase, CYP2C9 and CYP3A4 levels were significantly (P<0.05) higher in the mid-late phase when compared with the early phase. CYP2C9, 2J2 and 3A4 proteins were each localized to the large luteal cells, with 2C9 and 2J2 also being present in the small luteal, stromal and endothelial cells. These studies demonstrate for the first time that an EpETrE generating and metabolizing system exists in the primate CL, with the latter being regulated by LH and steroid hormone(s).
dc.format application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CORPUS LUTEUM
dc.subject CYTOCHROME P-450 ENZIME
dc.subject EPOXIDE HYDROLOSES
dc.subject RNA MESSENGER
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-10-06T18:01:13Z
dc.identifier.eissn 1460-2407
dc.journal.volume 13
dc.journal.number 8
dc.journal.pagination 541-548
dc.journal.pais Reino Unido
dc.journal.ciudad Oxford
dc.description.fil Fil: Irusta, Griselda. State University of Oregon; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Murphy, M. J.. Oregon Health and Science University; Estados Unidos
dc.description.fil Fil: Perez, W. D.. Oregon Health and Science University; Estados Unidos
dc.description.fil Fil: Hennebold J.D.. Oregon Health and Science University; Estados Unidos
dc.journal.title Molecular Human Reproduction
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/molehr/article-lookup/doi/10.1093/molehr/gam044
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/molehr/gam044
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/pmid/17567599


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