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dc.contributor.author
Valenti, Laura Elisa
dc.contributor.author
Martins, Vitor L.
dc.contributor.author
Herrera, Elisa Gabriela
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Torresi, Roberto M.
dc.contributor.author
Giacomelli, Carla Eugenia
dc.date.available
2017-09-13T21:08:23Z
dc.date.issued
2013-07
dc.identifier.citation
Valenti, Laura Elisa; Martins, Vitor L.; Herrera, Elisa Gabriela; Torresi, Roberto M.; Giacomelli, Carla Eugenia; Ni(II)-modified solid substrates as a platform to adsorb His-tag proteins; Royal Society of Chemistry; Journal Of Materials Chemistry; 1; 38; 7-2013; 4921-4931
dc.identifier.issn
0959-9428
dc.identifier.uri
http://hdl.handle.net/11336/24215
dc.description.abstract
This work investigated a simple and versatile modification to a solid substrate to develop electrochemical bio-recognition platforms based on bio-affinity interactions between histidine (His)-tagged proteins and Ni(II) surface sites. Carboxylate (COO)-functionalized substrates were prepared in multiple steps, initiated with an amino-terminated self-assembled monolayer (SAM) on polycrystalline gold. Surface enhanced Raman spectroscopy (SERS), quartz crystal microbalance with dissipation monitoring (QCM-D) and contact angle measurements were used to follow the modification process. Upon completion of the modification process, the surface COO–Ni(II) chelate complex and the coordination mode used to bind the His-tag proteins were characterized by X-ray absorption near-edge spectroscopy (XANES). Finally, the electrochemical stability and response of the modified substrates were evaluated. The versatility of the modification process was verified using silica as the substrate. QCM-D and SERS results indicated that two types of films were formed: a COO-terminated SAM, which resulted from the reduction of previously incorporated surface aldehyde groups, and a physically adsorbed polymeric glutaraldehyde film, which was produced in the alkaline medium. XANES spectral features indicated that COO–Ni(II) formed a non-distorted octahedral complex on the substrate. The electrochemical stability and response towards a redox mediator of the COO–Ni(II)-terminated SAM indicated that this platform could be easily coupled to an electrochemical method to detect bio-recognition events.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Royal Society of Chemistry
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Otras Ciencias Químicas
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Ni(II)-modified solid substrates as a platform to adsorb His-tag proteins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-13T14:16:31Z
dc.journal.volume
1
dc.journal.number
38
dc.journal.pagination
4921-4931
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Valenti, Laura Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Martins, Vitor L.. Universidade de Sao Paulo; Brasil
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Fil: Herrera, Elisa Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Torresi, Roberto M.. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Giacomelli, Carla Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Journal Of Materials Chemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/c3tb20769b
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb20769b
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