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dc.contributor.author
Romero, Marcelo Ricardo
dc.contributor.author
Baruzzi, Ana Maria
dc.contributor.author
Garay, Fernando Sebastian
dc.date.available
2017-07-14T17:32:05Z
dc.date.issued
2011-12
dc.identifier.citation
Romero, Marcelo Ricardo; Baruzzi, Ana Maria; Garay, Fernando Sebastian; Mathematical modeling and experimental results of a sandwich-type amperometric biosensor; Elsevier Science; Sensors and Actuators B: Chemical; 162; 1; 12-2011; 284-291
dc.identifier.issn
0925-4005
dc.identifier.uri
http://hdl.handle.net/11336/20600
dc.description.abstract
A comprehensive numerical treatment of the diffusion and reaction within a sandwich-type amperometric biosensor is presented. The model considers that the enzyme reacts according to a ping-pong mechanism and that it is entrapped into a so-called enzymatic matrix placed between two diffusion membranes. It is found that the concentrations of reagents and products within the sensor are difficult of being compared to those of the bulk. In this regard, the use of approximate analytical solutions would involve errors in the analysis of kinetic parameters corresponding to this kind of biosensors. Provided the mediator species are in high concentration or diffuse much faster than the substrate, the response time of a biosensor of this kind would be determined by the diffusion of the substrate though the external membrane. In this sense, those systems with immobilized mediators, in which diffusion of electrons or holes is assumed for the charge transport process, could be also described by this model. Thus, the thickness and the permeability to the analyte of the external membrane are critical parameters for improving the response time of a sandwich-type biosensor. The simulated curves are compared with experimental profiles corresponding to a lactate amperometric biosensor obtaining consistent results. In a future publication a non-linear fitting algorithm will be combined to the model for the extraction of kinetic and/or geometric parameters.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Biosensors
dc.subject
Polymers
dc.subject
Lactate
dc.subject
Simulation
dc.subject.classification
Físico-Química, Ciencia de los Polímeros, Electroquímica
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Mathematical modeling and experimental results of a sandwich-type amperometric biosensor
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T18:30:45Z
dc.journal.volume
162
dc.journal.number
1
dc.journal.pagination
284-291
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Romero, Marcelo Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Baruzzi, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.description.fil
Fil: Garay, Fernando Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
dc.journal.title
Sensors and Actuators B: Chemical
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.snb.2011.12.079
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0925400511011695
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