Mostrar el registro sencillo del ítem
dc.contributor.author
Galera, Ivana Laura Delia
dc.contributor.author
Paraje, Maria Gabriela
dc.contributor.author
Paez, Paulina Laura
dc.date.available
2017-06-30T14:16:25Z
dc.date.issued
2016-02-18
dc.identifier.citation
Galera, Ivana Laura Delia; Paraje, Maria Gabriela; Paez, Paulina Laura; Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria; International Knowledge Press; Journal of Biology and Nature; 5; 3; 18-2-2016; 2395-5376
dc.identifier.issn
2395-5376
dc.identifier.uri
http://hdl.handle.net/11336/19246
dc.description.abstract
Oxygen is essential to the life of the aerobic organisms, and most is used for the intracellular generation of energy, where several molecules are formed by partial reduction of oxygen. Because of its short half-life, low selectivity and high reactivity, biological targets of reactive oxygen species (ROS) include various components such as proteins, nucleic acids and lipids. The purpose of this work was to establish a relationship between ROS and reactive nitrogen intermediates (RNI) generation in bacteria exposed to antibiotics. ROS was determined by the reduction of nitro blue tetrazolium (NBT). In aqueous solutions, nitric oxide is converted to nitrite (NO2-), this was determined using a non-enzymatic colorimetric assay. NBT studies indicated stimulation of oxidative stress with an increase of ROS generated by the antibiotic in the bacteria. We observed an increase in the production of NO2- dose-dependent with CIP and GEN. The determination of ROS and RNI allowed establish differences in the alteration of oxidative metabolism in susceptible and resistant strains. Resistance to CIP and GEN may be related to the decreased production of ROS and RNI and consequently, a smaller macromolecules bacterial oxidation. These results contribute to a better understanding of the ROS and RNI regulation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
International Knowledge Press
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oxidative Stress
dc.subject
Bacteria
dc.subject
Nitrosative Stress
dc.subject
Antibiotics
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Relationship between oxidative and nitrosative stress induced by gentamicin and ciprofloxacin in bacteria
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-06-29T13:55:35Z
dc.identifier.eissn
2395-5384
dc.journal.volume
5
dc.journal.number
3
dc.journal.pagination
2395-5376
dc.journal.pais
India
dc.journal.ciudad
India
dc.description.fil
Fil: Galera, Ivana Laura Delia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina
dc.description.fil
Fil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Paez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Biology and Nature
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ikpress.org/abstract/5319
Archivos asociados