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dc.contributor.author
Andrade, Grazielle S. S.
dc.contributor.author
Carvalho, Ana K. F.
dc.contributor.author
Romero, Cintia Mariana
dc.contributor.author
Oliveira, Pedro C.
dc.contributor.author
Castro, Heizir F. de
dc.date.available
2017-05-31T18:55:12Z
dc.date.issued
2014-06
dc.identifier.citation
Andrade, Grazielle S. S.; Carvalho, Ana K. F.; Romero, Cintia Mariana; Oliveira, Pedro C.; Castro, Heizir F. de; Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil; Springer; Bioprocess And Biosystems Engineering; 37; 12; 6-2014; 2539-2548
dc.identifier.issn
1615-7591
dc.identifier.uri
http://hdl.handle.net/11336/17186
dc.description.abstract
The intracellular lipase production by Mucor circinelloides URM 4182 was investigated through a step-by-step strategy to attain immobilized whole-cells with high lipase activity. Physicochemical parameters, such as carbon and nitrogen sources, inoculum size and aeration, were studied to determine the optimum conditions for both lipase production and immobilization in polyurethane support. Olive oil and soybean peptone were found to be the best carbon and nitrogen sources, respectively, to enhance the intracellular lipase activity. Low inoculum level and poor aeration rate also provided suitable conditions to attain high lipase activity (64.8 +/- A 0.8 U g(-1)). The transesterification activity of the immobilized whole- cells was assayed and optimal reaction conditions for the ethanolysis of babassu oil were determined by experimental design. Statistical analysis showed that M. circinelloides whole-cells were able to produce ethyl esters at all tested conditions, with the highest yield attained (98.1 %) at 35 A degrees C using an 1:6 oil-to-ethanol molar ratio. The biocatalyst operational stability was also assayed in a continuous packed bed reactor (PBR) charged with glutaraldehyde (GA) and Aliquat-treated cells revealing half-life of 43.0 +/- A 0.5 and 20.0 +/- A 0.8 days, respectively. These results indicate the potential of immobilized M. circinelloides URM 4182 whole-cells as a low-cost alternative to conventional biocatalysts in the production of ethyl esters from babassu oil. (AU)
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lipase
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Mucor Circinelloide
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Immobilized Whole-Cells
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Biotalaysis
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Ethyl Esters
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Polyurethane
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Mucor circinelloides whole-cells as a biocatalyst for the production 3 of ethyl esters based on babassu oil
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-02-05T14:59:58Z
dc.journal.volume
37
dc.journal.number
12
dc.journal.pagination
2539-2548
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Andrade, Grazielle S. S.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
dc.description.fil
Fil: Carvalho, Ana K. F.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
dc.description.fil
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
dc.description.fil
Fil: Oliveira, Pedro C.. Universidade de Sao Paulo. Instituto de Biociências; Brasil
dc.description.fil
Fil: Castro, Heizir F. de. Universidade de Sao Paulo. Instituto de Biociências; Brasil
dc.journal.title
Bioprocess And Biosystems Engineering
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00449-014-1231-4
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00449-014-1231-4
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