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dc.contributor.author
Wertheimer Hermitte, Eva Victoria
dc.contributor.author
Krapf, Dario
dc.contributor.author
de la Vega Beltran, José L.
dc.contributor.author
Sánchez Cárdenas, Claudia
dc.contributor.author
Navarrete, Felipe
dc.contributor.author
Haddad, Douglas
dc.contributor.author
Escoffier, Jessica
dc.contributor.author
Salicioni, Ana M.
dc.contributor.author
Levin, Lonny R.
dc.contributor.author
Buck, Jochen
dc.contributor.author
Mager, Jesse
dc.contributor.author
Darszon, Alberto
dc.contributor.author
Visconti, Pablo E.
dc.date.available
2017-03-14T14:25:03Z
dc.date.issued
2013-12
dc.identifier.citation
Wertheimer Hermitte, Eva Victoria; Krapf, Dario; de la Vega Beltran, José L.; Sánchez Cárdenas, Claudia; Navarrete, Felipe; et al.; Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.; American Society for Biochemistry and Molecular Biology; Journal Of Biological Chemistry (online); 288; 49; 12-2013; 35307-35320
dc.identifier.uri
http://hdl.handle.net/11336/13822
dc.description.abstract
Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Biochemistry and Molecular Biology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Acrosome Reaction
dc.subject
Adenilate Cyclase (Adenylyl Cyclase)
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Calcium Imaging
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Cell Signaling
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Cyclic Amp (Camp)
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Forskolin
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Heterotrimeric G Proteins
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Protein Kinase a (Pka)
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Signal Transduction
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Sperm Capacitation
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-06T15:25:16Z
dc.identifier.eissn
1083-351X
dc.journal.volume
288
dc.journal.number
49
dc.journal.pagination
35307-35320
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina
dc.description.fil
Fil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Navarrete, Felipe. University Of Massachussets; Estados Unidos
dc.description.fil
Fil: Haddad, Douglas. University Of Massachussets; Estados Unidos
dc.description.fil
Fil: Escoffier, Jessica. University Of Massachussets; Estados Unidos
dc.description.fil
Fil: Salicioni, Ana M.. University Of Massachussets; Estados Unidos
dc.description.fil
Fil: Levin, Lonny R.. Cornell University; Estados Unidos
dc.description.fil
Fil: Buck, Jochen. Cornell University; Estados Unidos
dc.description.fil
Fil: Mager, Jesse. University Of Massachussets; Estados Unidos
dc.description.fil
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Visconti, Pablo E.. University Of Massachussets; Estados Unidos
dc.journal.title
Journal Of Biological Chemistry (online)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.jbc.org/content/early/2013/10/15/jbc.M113.489476
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1074/jbc.M113.489476
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