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dc.contributor.author
Falcón Dieguez, Jose
dc.contributor.author
Rodi, Pablo Marcelo
dc.contributor.author
Lores Guevara, Manuel
dc.contributor.author
Gennaro, Ana Maria
dc.date.available
2017-03-06T18:20:32Z
dc.date.issued
2010-05
dc.identifier.citation
Falcón Dieguez, Jose; Rodi, Pablo Marcelo; Lores Guevara, Manuel; Gennaro, Ana Maria; Spin Label Studies of the Hemoglobin–Membrane Interaction During Sickle Hemoglobin Polymerization; Springer Wien; Applied Magnetic Resonance; 38; 4; 5-2010; 443-453
dc.identifier.issn
0937-9347
dc.identifier.uri
http://hdl.handle.net/11336/13561
dc.description.abstract
An enhanced hemoglobin–membrane association has been previously documented in sickle cell anemia. However, it is not known how this interaction is modified during the hemoglobin S polymerization process. In this work, we use a model of reconstituted erythrocytes from ghost membranes whose cytoskeleton proteins had been previously labeled with the 4-maleimido Tempo spin label, and that were subsequently resealed with hemoglobin S or A solutions. Using electron paramagnetic resonance spectroscopy, we studied the time dependence of the spectral W/S parameter, indicative of the conformational state of cytoskeleton proteins (mainly spectrin) under spontaneous deoxygenation, with the aim of detecting the eventual effects due to hemoglobin S polymerization. The differences observed in the temporal behavior of W/S in erythrocytes reconstituted with both hemoglobins were considered as experimental evidence of an increment in hemoglobin S–membrane interaction as a result of the polymerization process of hemoglobin S under spontaneous deoxygenation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Wien
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hemoglobin-Membrane Interaction
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Hemoglobin S Polymerization
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Reconstituted Erythrocytes
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4-Maleimido Tempo Spin Label
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Spin Label Studies of the Hemoglobin–Membrane Interaction During Sickle Hemoglobin Polymerization
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-03-01T17:49:30Z
dc.journal.volume
38
dc.journal.number
4
dc.journal.pagination
443-453
dc.journal.pais
Austria
dc.description.fil
Fil: Falcón Dieguez, Jose. Universidad de Oriente; Cuba
dc.description.fil
Fil: Rodi, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Lores Guevara, Manuel. Universidad de Oriente; Cuba
dc.description.fil
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.journal.title
Applied Magnetic Resonance
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00723-010-0138-8
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