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dc.contributor.author
Caballero, Veronica Jorgelina
dc.contributor.author
Mendieta, Julieta Renee
dc.contributor.author
Lombardo, Daniel
dc.contributor.author
Saceda, Miguel
dc.contributor.author
Ferragut, José Antonio
dc.contributor.author
Conde, Ruben Danilo
dc.contributor.author
Giudici, Ana Marcela
dc.date.available
2017-02-16T13:55:29Z
dc.date.issued
2014-11
dc.identifier.citation
Caballero, Veronica Jorgelina; Mendieta, Julieta Renee; Lombardo, Daniel; Saceda, Miguel; Ferragut, José Antonio; et al.; Liver damage and caspase-dependent apoptosis is related to protein
malnutrition in mice: Effect of methionine; Elsevier Gmbh; Acta Histochemica; 117; 1; 11-2014; 126-135
dc.identifier.issn
0065-1281
dc.identifier.uri
http://hdl.handle.net/11336/13066
dc.description.abstract
This study aimed to determine whether the effects on the mouse liver caused by three periods of feeding a protein-free diet for 5 days followed by a normal complete diet for 5 days (3PFD-CD) are prevented by a constant methionine supply (3PFD + Met-CD). The expressions of carbonic anhydrase III (CAIII), fatty acid synthase (FAS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glutathione S-transferase P1 (GSTP1) were assessed by proteomics and reverse transcriptase-polymerase chain reactions. The liver redox status was examined by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein carbonylation. Because oxidative stress can result in apoptosis, the activity and content of caspase-3, as well as the x-linked inhibitor of the apoptosis protein (XIAP) and mitochondrial caspase-independent apoptosis inducing factor (AIF) contents were assessed. In addition, the liver histomorphology was examined. Compared to the controls fed a normal complete diet throughout, feeding with 3PFD-CD increased the FAS content, decreased the CAIII content, decreased both the SOD and CAT activities, and increased protein carbonylation. It also activated caspase-3, decreased the XIAP content, decreased the AIF content, increased the number of GSTP1-positive foci and caspase-3-positive cells, and caused fatty livers. Conversely, the changes were lessened to varying degrees in mice fed 3PFD + Met-CD. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Gmbh
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Apoptosis
dc.subject
Protein Depletion
dc.subject
Liver
dc.subject
Methionine
dc.subject
Mice
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Liver damage and caspase-dependent apoptosis is related to protein
malnutrition in mice: Effect of methionine
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-10T18:13:29Z
dc.journal.volume
117
dc.journal.number
1
dc.journal.pagination
126-135
dc.journal.pais
Alemania
dc.journal.ciudad
Munich
dc.description.fil
Fil: Caballero, Veronica Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil
Fil: Mendieta, Julieta Renee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil
Fil: Lombardo, Daniel. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
dc.description.fil
Fil: Saceda, Miguel. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España
dc.description.fil
Fil: Ferragut, José Antonio. Universidad de Miguel Hernandez. Centro de Biologia Molecular y Celular; España
dc.description.fil
Fil: Conde, Ruben Danilo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.description.fil
Fil: Giudici, Ana Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
dc.journal.title
Acta Histochemica
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0065128114002888
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.acthis.2014.11.012
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