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dc.contributor.author Sangorrin, Marcela Paula
dc.contributor.author García, V.
dc.contributor.author Lopes, Christian Ariel
dc.contributor.author Saez, Julieta Susana
dc.contributor.author Martínez, C.
dc.contributor.author Ganga, M. A.
dc.date.available 2017-01-19T21:06:15Z
dc.date.issued 2013-04
dc.identifier.citation Sangorrin, Marcela Paula; García, V.; Lopes, Christian Ariel; Saez, Julieta Susana; Martínez, C.; et al.; Molecular and physiological comparison of spoilage wine yeasts; Wiley; Journal of Applied Microbiology; 114; 4; 4-2013; 1066-1074
dc.identifier.issn 1364-5072
dc.identifier.uri http://hdl.handle.net/11336/11677
dc.description.abstract Aims: Dekkera bruxellensis and Pichia guilliermondii are contaminating yeasts in wine due to the production of phenolic aromas. Although the degradation pathway of cinnamic acids, precursors of these phenolic compounds has been described in D. bruxellensis, no such pathway has been described in P. guilliermondii. Methods and Results: A molecular and physiological characterization of 14 D. bruxellensis and 15 P. guilliermondii phenol-producing strains was carried out. Both p-coumarate decarboxylase (CD) and vinyl reductase (VR) activities, responsible for the production of volatile phenols, were quantified and the production of 4-VP and 4-EP were measured. All D. bruxellensis and some P. guilliermondii strains showed the two enzymatic activities, whilst 11 of the 15 strains of this latter species showed only CD activity and did not produce 4-EP in the assay conditions. Furthermore, PCR products obtained with degenerated primers showed a low homology with the sequence of the gene for a phenyl acrylic acid decarboxylase activity described in Saccharomyces cerevisiae. Conclusions: D. bruxellensis and P. guilliermondii may share a similar metabolic pathway for the degradation of cinnamic acids. Significance and Impact of Study. This is the first work that analyses the CD and VR activities in P. guilliermondii and the results suggest that within this species there are differences in the metabolization of cinnamic acids.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject DEKKERA BRUXELLENSIS
dc.subject OFF-FLAVOUR
dc.subject PHENOLS
dc.subject PICHIA GUILLIERMONDII
dc.subject WINE
dc.subject.classification Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
dc.subject.classification Biotecnología Industrial
dc.subject.classification INGENIERÍAS Y TECNOLOGÍAS
dc.title Molecular and physiological comparison of spoilage wine yeasts
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-01-17T19:10:46Z
dc.identifier.eissn 1365-2672
dc.journal.volume 114
dc.journal.number 4
dc.journal.pagination 1066-1074
dc.journal.pais Reino Unido
dc.journal.ciudad Londres
dc.description.fil Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
dc.description.fil Fil: García, V.. Universidad de Santiago de Chile; Chile
dc.description.fil Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
dc.description.fil Fil: Saez, Julieta Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
dc.description.fil Fil: Martínez, C.. Universidad de Santiago de Chile; Chile
dc.description.fil Fil: Ganga, M. A.. Universidad de Santiago de Chile; Chile
dc.journal.title Journal of Applied Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12134/abstract
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/jam.12134


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)